E-cadherin mediated cell-cell adhesion plays a critical role in epithelial cell polarization and morphogenesis. Our recent studies suggest that the desmosomal cadherin, desmoglein 3 (Dsg3) cross talks with E-cadherin and regulates its adhesive function in differentiating keratinocytes. However, the underlying mechanism remains not fully elucidated. Since E-cadherin trafficking has been recognized to be a central determinant in cell-cell adhesion and homeostasis we hypothesize that Dsg3 may play a role in regulating E-cadherin trafficking and hence the cell-cell adhesion. Here we investigated this hypothesis in cells with loss of Dsg3 function through RNAi mediated Dsg3 knockdown or the stable expression of the truncated mutant Dsg3DC. Our results showed that loss of Dsg3 resulted in compromised cell-cell adhesion and reduction of adherens junction and desmosome protein expression as well as the cortical F-actin formation. As a consequence, cells failed to polarize but instead displayed aberrant cell flattening. Furthermore, retardation of Ecadherin internalization and recycling was consistently observed in these cells during the process of calcium induced junction assembling. In contrast, enhanced cadherin endocytosis was detected in cells with overexpression of Dsg3 compared to control cells. Importantly, this altered cadherin trafficking was found to be coincided with the reduced expression and activity of Rab proteins, including Rab5, Rab7 and Rab11 which are known to be involved in E-cadherin trafficking. Taken together, our findings suggest that Dsg3 functions as a key in cell-cell adhesion through at least a mechanism of regulating E-cadherin membrane trafficking.
نتيجة لما ابداه الخبراء الطبيون الليبيون والدوليون من مخاوف بسبب احتمال حدوث زيادات في العدوى المرضية، وخاصة التهاب الكبد الفيروسي. وبسبب تفشي فيروساتها بالكبد B و C وعوامل الخطر بين السكان الليبيين ولان الدراسات غير متوفرة عليه، فإن هذه الدراسة تهدف إلى دراسة نتائج تحليل عينات الدم بين الليبيين في مختبر الهلال الأحمر في مدينة المرج-ليبيا.أجريت هذه الدراسة في الفترة من يناير 2014 حتى نهاية ديسمبر 2016 وشملت عددا من 12000 حالة من المتقدمين للحصول على شهادة صحية لأغراض مختلفة. وجد ان 63.5٪ (ن = 7596)، 36.5٪ (4404 =ن) من الذكور والإناث على التوالي. تم مسح نتائج عينات الدم للكشف عن وجود عدوى بفيروس الكبد B و C باستخدام فحص المناعي المرتبط الانزيم (إليزا).ومن بين ما مجموعه 12000 عينة.كان متوسط انتشار الفيروس بي بنسبة 0.4٪ ومضاد سى بنسبة 1٪. كان فيروساتها بالكبد الوبائي أكثر انتشارا بين الذين تتراوح أعمارهم بين 27-37 عاما. وكانت نسبة سي بين أولئك الذين تتراوح أعمارهم بين 16-26 سنة. وخلصت النتائج إلى أن العدوى بفيروس بي و سي بين الفئات العمرية الأصغر سنا أصبحت قضية مثيرة للقلق. ويتعين تنفيذ اللوائح والتعاليم في مجال الرعاية الصحية، وينبغي التخطيط للمتابعة على المدى الطويل.
Ferritin, a major iron storage protein, is essential to iron homeostasis and is involved in a wide range of physiologic and pathologic processes, and can hold up to 4 000 atoms of iron. The aim of our study was to determine the levels of Ferritin among different age groups in El-Beyda city in women of childbearing age. This study included 217 women in childbearing age (18- 45 years) who were visiting women clinic and in a period of 24 months from the first of January 2016 to December 2017in El-Beyda city. Reliable serum ferritin level was measured by using an enzyme immunoassay ELISA method. This study showed that there is a close relationship between age and level of serum ferritin, where we found that the age groups 25-31 and 32-38 is the lowest in the level of serum ferritin.
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