Background. Pulp tissue affected by deep caries and trauma can be protected by vital pulp therapies in which pulp regeneration success depends on the degree of pulp inflammation and the presence of regenerative signals. Reparative dentinogenesis requires dental pulp stem cell (DPSC) activity which can be stimulated by many bioactive molecules to repair the dentine, mediating a balance between the inflammatory response and the reparative events. Therefore, this study was performed in order to investigate the immune-inflammatory effect of Biodentine capping material on DPSCs and macrophages. Method. THP-1, a human monocytic cell line, was differentiated to macrophages, and flow cytometry was used to analyze the expressions of specific macrophage markers. LPS-mediated infection was created for macrophages and DPSCs followed by treatment with Biodentine. CBA array was used to investigate the cytokine secretion followed by qPCR. Migration potential of treated DPSCs was also determined. Results. Our results showed that THP-1 cell line was successfully differentiated into macrophages as shown by surface marker expression. CBA array and qPCR results showed that Biodentine-treated DPSCs and macrophages upregulated anti-inflammatory cytokines and downregulated proinflammatory cytokines. Also, Biodentine enhances the migration potential of treated DPSCs. Conclusion. Biodentine capping material mediated the polarization of M1 to M2 macrophages suggestive of tissue repair properties of macrophages and enhanced the anti-inflammatory cytokines of DPSCs responsible for dentine-pulp regeneration.
This paper aims to investigate greenhouse gas emissions (GHGEs) in conflict areas, emphasising Palestine. This estimation faces several difficulties, particularly in data collection. The paper first presents the geopolitical context of Palestine and its consequences for the analysis of GHGEs. Then, it presents the estimation of the GHGEs related to energy, which constitutes the major contributor to GHGEs in Palestine. The emissions were estimated according to Tier 1 in the 2006 IPCC guidelines. For the first time in Palestine, (i) the International Energy Agency methodology for calculating emission factors from electricity consumption is used to estimate the emission factors of GHG at the final point of consumption, and (ii) the scope 2 emissions from imported electricity are accounted for in the total emissions. The GHGEs from the Palestinian electricity generation (excluding imported electricity) were 446,471 tons of CO2eq in 2019, representing 11% of the total emissions from the energy sector. The total GHGEs from electricity final consumption (including imported electricity) in all sectors were 3,929,829 tons of CO2eq. More than 60% (2,316,465 tons of CO2eq) of these emissions were attributed to the consumption of electricity by the households. Emissions from fuel consumption in 2019 were about 3,912,566 tons of CO2eq. Transport was found to be the main emitter of GHGs, with more than half of the total emissions (2,207,834 tons of CO2eq). Considering imported electricity in estimating the total GHGEs from the energy sector doubled the emissions. It increased the share of household emissions in the total GHGEs from the energy sector to become the most significant contributor to the total emissions.
The transport sector is the second leading emitter of greenhouse gas emissions (GHGEs) from fuel combustion activities on a global scale. Reducing emissions related to this sector requires detailed data about the emissions by vehicle type and traveled distance. Generally, these data are missing in developing countries, which makes difficult the establishment of effective policies for the reduction of these emissions. This paper presents a hybrid method to estimate these emissions using the IPCC 2006 guidelines. The method combines bottom-up and top-down approaches to estimate vehicular emissions using data about the vehicle type, vehicle kilometers traveled, and fuel consumption. This method is applied for the first time for the Palestinian territory. Data have been collected from the administration, official reports, and papers. The results show a significant increase in the total vehicles in Palestine, particularly diesel vehicles. Emissions from the on-road transport system were approximately 2,207,834 tons of CO2eq in 2019. Diesel vehicles were responsible for about 75% of these emissions. Private cars were the most significant contributor to these emissions, with a share exceeding 50% of the total emissions, followed by commercial vehicles and light trucks (20%), public taxis (9%), and buses (7%). These results show clearly that the GHGEs reduction policies in Palestine should focus on diesel and private vehicles by developing the public transport systems and replacing diesel and gasoline vehicles with more environmentally friendly vehicles, such as hybrid and electric cars.
Background & objectives: Platelet concentrate contains a rich cocktail of growth factors that support growth and proliferation of cells. The primary goal of this study was to investigate the safety of platelet lysate (PL) in patients with critical limb ischaemia (CLI) not suitable for standard revascularization. Data on the preliminary efficacy are also presented. Methods: Seven patients (18-70 yr) with CLI classified in the Rutherford grades 3-5, with history of intermittent claudication for more than eight weeks and were not suitable for standard revascularization, underwent autologous intramuscular injections of PL. These patients were examined physically alongside other parameters such as TcPO2, toe pressure, and ankle brachial index, at baseline and were followed up for 12 months. Results: The procedure was well tolerated with no serious adverse or any adverse events reported during follow up. Although not the primary aim of this report, preliminary data showed significant clinical improvement in Rutherford stage, ankle-brachial index and toe pressure which persisted for a year. Interpretation & conclusions: Intramuscular injection of PL was well tolerated with no major adverse events reported in our study participants. With the observed satisfactory safety data, preliminary efficacy data of PL should be further validated.
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