HighlightRecent research shows that sugar availability triggers bud outgrowth. This paper further demonstrates that the effect of sucrose involves changes in the hormonal network related to bud outgrowth, and identifies potential hormones involved in sugar control.

Bud outgrowth is controlled by environmental and endogenous factors. Through the use of the photosynthesis inhibitor norflurazon and of masking experiments, evidence is given here that light acts mainly as a morphogenic signal in the triggering of bud outgrowth and that initial steps in the light signaling pathway involve cytokinins (CKs). Indeed, in rose (Rosa hybrida), inhibition of bud outgrowth by darkness is suppressed solely by the application of CKs. In contrast, application of sugars has a limited effect. Exposure of plants to white light (WL) induces a rapid (after 3-6 h of WL exposure) up-regulation of CK synthesis (RhIPT3 and RhIPT5), of CK activation (RhLOG8), and of CK putative transporter RhPUP5 genes and to the repression of the CK degradation RhCKX1 gene in the node. This leads to the accumulation of CKs in the node within 6 h and in the bud at 24 h and to the triggering of bud outgrowth. Molecular analysis of genes involved in major mechanisms of bud outgrowth (strigolactone signaling [RwMAX2], metabolism and transport of auxin [RhPIN1, RhYUC1, and RhTAR1], regulation of sugar sink strength [RhVI, RhSUSY, RhSUC2, and RhSWEET10], and cell division and expansion [RhEXP and RhPCNA]) reveal that, when supplied in darkness, CKs up-regulate their expression as rapidly and as intensely as WL. Additionally, up-regulation of CKs by WL promotes xylem flux toward the bud, as evidenced by Methylene Blue accumulation in the bud after CK treatment in the dark. Altogether, these results suggest that CKs are initial components of the light signaling pathway that controls the initiation of bud outgrowth.

Branching determines the final shape of plants, which influences adaptation, survival and the visual quality of many species. It is an intricate process that includes bud outgrowth and shoot extension, and these in turn respond to environmental cues and light conditions. Light is a powerful environmental factor that impacts multiple processes throughout plant life. The molecular basis of the perception and transduction of the light signal within buds is poorly understood and undoubtedly requires to be further unravelled. This review is based on current knowledge on bud outgrowth-related mechanisms and light-mediated regulation of many physiological processes. It provides an extensive, though not exhaustive, overview of the findings related to this field. In parallel, it points to issues to be addressed in the near future.

Bud outgrowth is a key process in the elaboration of yield and visual quality in rose crops. Although light intensity is well known to affect bud outgrowth, little is known on the mechanisms involved in this regulation. The objective of this work was to test if the control of bud outgrowth pattern along the stem by photosynthetic photon flux density (PPFD) is mediated by sugars, cytokinins and/or abscisic acid in intact rose plants. Rooted cuttings of Rosa hybrida ‘Radrazz’ were grown in growth chambers under high PPFD (530 μmol m-2 s-1) until the floral bud visible stage. Plants were then either placed under low PPFD (90 μmol m-2 s-1) or maintained under high PPFD. Bud outgrowth inhibition by low PPFD was associated with lower cytokinin and sugar contents and a higher abscisic acid content in the stem. Interestingly, cytokinin supply to the stem restored bud outgrowth under low PPFD. On the other hand, abscisic acid supply inhibited outgrowth under high PPFD and antagonized bud outgrowth stimulation by cytokinins under low PPFD. In contrast, application of sugars did not restore bud outgrowth under low PPFD. These results suggest that PPFD regulation of bud outgrowth in rose involves a signaling pathway in which cytokinins and abscisic acid play antagonistic roles. Sugars can act as nutritional and signaling compounds and may be involved too, but do not appear as the main regulator of the response to PPFD.

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Bud outgrowth is under the intricate control of environmental and endogenous factors. In a recent paper, we demonstrated that light perceived by Rosa buds triggers cytokinins (CK) synthesis within 3 hours in the adjacent node followed by their transport to the bud. There, CK control expression of a set of major genes (strigolactones-, auxin-, sugar sink strength-, cells division and elongation-related genes) leading to bud outgrowth in light. Conversely, under dark condition, CK accumulation and transport to the bud are repressed and no bud outgrowth occurs. In this paper, we show that the 3 expansin genes RhEXPA1,2,3 are under the control of both light and CK during bud outgrowth. In silico analysis of promoter sequences highlights 2 regions enriched in light and CK cis-regulatory elements as well as a specific cis-element in pRhEXPA3, potentially responsible for the expression patterns observed in response to CK and light.

To integrate the gene pool of a wild species (primarily diploid) into a cultivated pool (primarily tetraploid), a crossing between a dihaploid cultivated rose and a hybrid of Rosa wichurana allowed to obtain interspecific diploid hybrids that produced 2n pollen grains. A return to a tetraploid level sought by breeders can then be considered using sexual polyploidization, obtained by crossing a tetraploid cultivated rose with these hybrids. Application of a high-temperature regime led to a small but significant increase in the percentage of 2n pollen grains in these hybrids of up to 4.6%. This result was obtained by applying high temperatures close to 32°C during the day to plants cultivated in a glasshouse during recurrent cycles of bloom. Crosses were made between an unreleased tetraploid hybrid tea rose, as a female, and the diploid hybrid that produces the most 2n pollen grains. Tetraploid (42.1%) and triploid (57.9%) offspring were obtained. The use of these 2n pollen grains of the first division restitution type should facilitate the introgression of complex traits of interest. Key words: 2n gametesRose is the most economically important ornamental crop worldwide because of its popularity as a garden, landscape and potted plant, and as cut flower (Gudin 2000). The genus Rosa subgenus Rosa comprises ten sections and more than 200 species (Wissemann 2003, Foug ere-Danezan et al. 2015. Ma€ ıa and Venard (1976) and Berninger (1992) showed that <10 species belonging to three sections are at the origin of roses (Rosa hybrida L.) cultivated today. Many cultivated roses are tetraploid (2n = 4x = 28), whereas many wild species are generally diploid (2n = 2x = 14).Rose breeders have attempted to enrich the variability of the gene pool of cultivated roses by introducing germplasm of new diploid wild species. Many interspecific hybridizations between tetraploid rose cultivars and wild diploid species were carried out, but produced triploids that were generally sterile, making it very difficult to generate additional generations (Wylie 1955). To integrate the variability of diploid species into the gene pool of cultivated roses, a haploidization programme of cultivated roses was developed in the 1990s by Meynet et al. (1994) that consisted of reducing the ploidy level of cultivated rose from tetraploid to diploid. This programme involved three steps: (i) producing dihaploids (diploid) from tetraploid rose cultivars, (ii) creating progeny of interspecific diploid hybrids developed by crossing dihaploid cultivated rose cultivars with wild diploid species and (iii) returning to the tetraploid level (ploidy level most frequently encountered in the cultivated rose) (Crespel and Meynet 2003).Only the first two steps of this programme were carried out. Dihaploids of tetraploid rose cultivars were obtained by parthenogenesis in situ induced by irradiated pollen and immature embryo rescue techniques in vitro (Meynet et al. 1994). The study of their gametogenesis revealed different meiotic anomalies such as abnormal spind...