The cytotoxicity of the methanol extract of Origanum syriacum against human cerivical adenocarcinoma, Hela cells, was evaluated by the MTT assay. From the crude extract, a new flavone glycoside, acacetin-7-O-[2´´-O-α-L-rhamnopyranosyl-6´´-O-β -D-glucopyranosyl]-β -D-glucopyranoside (1), together with the ten known flavonoids luteolin (2), apigenin (3), luteolin-6-C-glucoside (4), luteolin-3´-methylether-6-C-glucoside (5), luteolin-7,4´-dimethyether-6-C-glucoside (6), apigenin-7- methylether-6-C-glucoside (7), apigenin-7-O-glucoside (8), diosmetin-7-O-glucoside (9), acacetin- 7-O-glucoside (10) and acacetin-7-O-rutinoside (11), was isolated from the methanol extract of the aerial parts of Origanum syriacum. The structure elucidation of the isolated compounds was performed by spectroscopic and chromatographic methods.
Compounds were isolated from a methanol extract of the dried stem barks of Viburnum sargentii Koehne. The structures of the compounds, namely 9'-O-methylvibsanol (3), furcatoside A (4) and lareciresinol (5) were elucidated by analysis of spectroscopic data and comparison with values for previously known analogues. In addition, (+)-catechin (1), (+)-epicatechin (2) were also isolated. This work also examined the cytotoxic effects of three compounds 3-5 (25-100 µM) in MCF-7 and A549 cells after 24, 48 and 72 h of exposure. Our results showed that 9'-O-methylvibsanol (3) exhibited strong concentration-dependent anticancer effects according to the MTT assay and produced morphological changes consistent with apoptosis, as confirmed by Ho3342 staining analysis revealed that more apoptotic cells were observed after 9'-O-methylvibsanol (3) treatment.
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