In this study, screening of Artemisia dracunculus accessions was investigated under water deficit based on physiological and phytochemical traits. The results clearly indicated that water deficit significantly reduced the relative water content, chlorophyll, and carotenoid contents and increased malondialdehyde, electrolyte leakage, and antioxidant activities. The responses of tarragon accessions to water deficit, however, were inconsistent. The HPLC analysis revealed the presence of chlorogenic, syringic, ferulic, vanillic, chicoric, and p-coumaric acids as major phenolic acids, while quercetin and herniarin were detected as the predominant flavonoid and coumarin compounds in the extracts. Our findings revealed that the water deficit not only increased the amounts of herniarin, luteolin, apigenin, caffeic acid, and syringic acid, but also introduced quercetin that was not present under normal conditions in Estahbanat. Nevertheless, these results were highly impacted by the accession type. The results indicated that Hamadan, Varamin and Estahbanat accessions could be introduced as tolerant accessions. Given the very different responses of tarragon accessions to water deficit and the diversity between these accessions, the findings of the present study could be an effective step in identifying and achieving homogeneous, drought-tolerant and high-yield potential accessions, and may help tarragon breeding programs as well as development of cultivation.
Chickpea and lentils are the two most economically important food legumes in dryland areas. They are traditionally sown in the spring of cold dryland areas of the Mediterranean regions. Therefore, the grain yield of the crop is affected by drought and high thermal stresses at the end of the season. Autumn cultivation of these crops by cold-tolerant varieties could increase grain yield up to 50%, then spring cultivation through higher availability of soil water. Breeding for cold-tolerant chickpea and lentil that is widely adaptable to autumn cultivation in cold regions and various growth conditions is the best strategic approach but requires a fine-tuned combination of advanced phenotyping and genotyping methods. However, breeding and selection of suitable cold-tolerant chickpea and lentil genotypes is complex by its narrow genetic base, which limits the sources of novel alleles. This chapter illustrates the morphological, physiological, and molecular effects of cold stress on chickpea and lentil growth and development. It will be also elaborated on conventional and advanced breeding approaches and application of advanced genotyping and phenotyping tools commonly used to develop cold-tolerant chickpea and lentil cultivars. The following, about key crop cold-tolerance traits that can be easily screened by using genotypic and phenotypic technologies are discussed.
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