ObjectivesMesenchymal stem cells have the ability to differentiate into various cell types, and thus have emerged as promising alternatives to chondrocytes in cell-based cartilage repair methods. The aim of this experimental study was to investigate the effect of bone marrow derived mesenchymal stem cells combined with platelet rich fibrin on osteochondral defect repair and articular cartilage regeneration in a canine model.MethodsOsteochondral defects were created on the medial femoral condyles of 12 adult male mixed breed dogs. They were either treated with stem cells seeded on platelet rich fibrin or left empty. Macroscopic and histological evaluation of the repair tissue was conducted after four, 16 and 24 weeks using the International Cartilage Repair Society macroscopic and the O’Driscoll histological grading systems. Results were reported as mean and standard deviation (sd) and compared at different time points between the two groups using the Mann-Whitney U test, with a value < 0.05 considered statistically significant.ResultsHigher cumulative macroscopic and histological scores were observed in stem cell treated defects throughout the study period with significant differences noted at four and 24 weeks (9.25, sd 0.5 vs 7.25, sd 0.95, and 10, sd 0.81 vs 7.5, sd 0.57; p < 0.05) and 16 weeks (16.5, sd 4.04 vs 11, sd 1.15; p < 0.05), respectively. Superior gross and histological characteristics were also observed in stem cell treated defects.ConclusionThe use of autologous culture expanded bone marrow derived mesenchymal stem cells on platelet rich fibrin is a novel method for articular cartilage regeneration. It is postulated that platelet rich fibrin creates a suitable environment for proliferation and differentiation of stem cells by releasing endogenous growth factors resulting in creation of a hyaline-like reparative tissue.Cite this article: D. Kazemi, K. Shams Asenjan, N. Dehdilani, H. Parsa. Canine articular cartilage regeneration using mesenchymal stem cells seeded on platelet rich fibrin: Macroscopic and histological assessments. Bone Joint Res 2017;6:98–107. DOI: 10.1302/2046-3758.62.BJR-2016-0188.R1.
Objective: To determine the frequency of Helicobacter pylori (HP) in patients with chronic dyspepsia referred to private laboratories in Mazar-i-Sharif city in 2019. Methods: This study is a descriptive cross-sectional study which the sampling method has performed randomly on six private laboratories in Mazari-Sharif city. The population under study consisted people with digestive disorders and a number of them had no any signs and symptoms. Subjects of study were persons that referred to serological and stool testing for HP at private laboratories in Mazar-e Sharif city in the winter season of 2019. Of course, the patients included in this study are mostly those who have been treated with gastritis tablets at one time but have not been cured. The variants of study included gender, age group, presence and absence of signs and symptoms, place of residence. The patients’ information was obtained through the patient record book and patient’s examination result sheet in which the blood serum and their stool were used for diagnosis. Results: From 108 patients were recruited for HP study, 53 people were men and 55 people women, who were used for diagnosis serologic and stool tests, which showed that 74% of patients had positive result for HP and 26% negative result for HP and these patients were between the ages of 15-65 in terms of presence and absence of sign and symptoms, 57% with loss of weight and stomach irritation, 14% with anorexia, 7% had headache and vertigo and 22% had no signs and symptoms. Conclusions: The present study on 108 who were referred to private laboratories for blood or stool examination were positive for male (39%) and negative (13%), for female (33%) were positive and (15%) negative. Likewise their age groups were different with the majority of patients being between the ages of 15-25 and the minority were 56-65 years. 57% of patient complaining gastric irritation, severe stomach pain and loss of weight. Keywords: Helicobacter pylori, gastritis, indigestion, dyspepsia, diagnostic tests, sensitivity, resistance.
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