Acute coronary syndrome caused by the rupture of atherosclerotic plaques is one of the primary causes of major cardiovascular events, and neovascularization within the plaque is closely associated with its stability. Long noncoding RNA (lncRNAs) is a type of noncoding RNA that serves a crucial role in regulating vascular endothelial cells (VECs). The aim of the present study was to investigate the effect of lncRNA TCONS_00024652 on the proliferation and angiogenesis of VECs following stimulation with TNF-α. The expression of lncRNA and miRNA was measured in human umbilical vein endothelial cells (HUVECs) by reverse transcription-quantitative polymerase chain reaction. Cell proliferation was measured using a Cell Counting Kit-8 assay. Wound healing and tube formation assays were performed to determine cell migration and angiogenesis. Interactions between TCONS_00024652 and miR-21 were determined using bioinformatics and a dual-luciferase reporter assay. The results demonstrated that TCONS_00024652 is highly expressed in TNF-α-induced HUVECs. Functional assays demonstrated that the dysregulated expression of TCONS_00024652 promotes endothelial cell proliferation and angiogenesis, whereas TCONS_00024652 knockdown induces the opposite effects. Bioinformatics analysis using starBase predicted putative binding at the 3′-untranslated region of TCONS_00024652 and miR-21 and luciferase reporter assays further verified this interaction. The results of the present study suggest that the targeting of TCONS_00024652 by miR-21 may be a potential method of improving vascular endothelial dysfunction, neovascularization maturation and plaque stabilization.
and BPG (34% increase), while the treatment with fibrin glue alone did not influence this parameter. BP with and without fibrin glue increased of 97% and 94% respectively the number of alkaline phosphatase (ALP) positive cells compared to the control. Finally BP determined the upregulation of transcription factors and component of the extracellular matrix. Conclusion:The present data show that BP has a high osteoinductive potential on human OBs, enhancing their activity. Objective: To evaluate the choice of microsurgical treatment modalities and its clinical effect on middle ear cholesteatoma and chronic suppurative otitis media.Methods: 110 cases with middle ear and mastoid lesions including middle ear cholesteatoma and chronic suppurative otitis media were analysed which performed canal wall up mastoidectomy or at the same time tympanoplasty and canal wall down mastoidotympanectomy depending on lesion extent and followed-up, observed the ear recovery, complications, recurrence and postoperative hearing improvement.Results: 110 cases including 66 cases of middle ear cholesteatoma, 44 cases of chronic suppurative otitis media, 51 canal wall up mastoidotympanectomy, 46 cases simultaneously followed by tympanoplasty, 59 canal wall down mastoidectomy, Intraoperative finding as followed auditory absence of bone destruction 36 cases including 7 cases of chronic suppurative otitis media, 29 cases of middle ear cholesteatoma; 7 cases of complete auditory ossicles including 4 cases of chronic suppurative otitis media, 3 cases of middle ear cholesteatoma; facial nerve canal bone destruction 22 cases, brain palate damaged and meningitis exposed in 13 cases. Conclusion:According to different lesions of middle ear and mastoid the specific disease in intraoperative, different operative methods can be used to obtain the corresponding clinical curative effect, the canal wall up mastoidectomy plus tympanoplasty, if indications mastered properly, the technical conditions permitted, can effectively keep the original middle ear mastoid anatomical structure and improve hearing skill, and this surgery is feasible; if tympanoplasty cannot be used to a wide range of middle ear cholesteatoma, canal wall down mastoidectomy should be preferred in order to avoid recurrence and affect the efficacy. Object: To study of simple congenital ossicular malformation clinical and audiological characteristics, and to discuss options to different auditory ossicles in ossicular chain reconstruction.Methods: Ossicular chain malformations in 75 cases (79 ears) were studied involving 43 males and 32 females, aged from 6 to 57 years old (average 23.5±14.5 years old). There are four cases of bilateral conductive deafness and 71 cases of unilateral conductive deafness (39 left ears and 32 right ears). Results of preoperative audiometry showed that air-bone gap was 38.7 ;dB of speech frequency. We operated exploratory tympanotomy in 79 ears (4 cases of bilateral). Among them, ossicular chain reconstruction was performed in 71 cases, while in six cases not do...
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