Arbuscular mycorrhizal (AM) fungi form endosymbioses with most plants, and they themselves are hosts for Mollicutes/Mycoplasma-related endobacteria (MRE). Despite their significance, genomic information for AM fungi and their MRE are relatively sparse, which hinders our understanding of their biology and evolution.We assembled the genomes of the AM fungus Diversispora epigaea (formerly Glomus versiforme) and its MRE and performed comparative genomics and evolutionary analyses.

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1The red raspberry, Rubus idaeus, is widely distributed in all temperate regions of Europe, Asia, 2 and North America and is a major commercial fruit valued for its taste, high antioxidant and 3 vitamin content. However, Rubus breeding is a long and slow process hampered by limited 4 genomic and molecular resources. Genomic resources such as a complete genome sequencing 5 and transcriptome will be of exceptional value to improve research and breeding of this high 6 value crop. Using a hybrid sequence assembly approach including data from both long and short 7 sequence reads, we present the first assembly of the Rubus idaeus genome (Joan J. variety). The 8 de novo assembled genome consists of 2,145 scaffolds with a genome completeness of 95.3% 9 and an N50 score of 638 KB. Leveraging a linkage map, we anchored 80.1% of the genome onto 10 seven chromosomes. Using over 1 billion paired-end RNAseq reads, we annotated 35,566 11 protein coding genes with a transcriptome completeness score of 97.2%. The Rubus idaeus 12 genome provides an important new resource for researchers and breeders. 13 14

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