A noninvasive method for quantifying hydration status would be helpful for clinical management and for research applications in dogs. This prospective, experimental, pilot study aimed to assess the feasibility of ultrasonographic measurement of the caudal vena cava to aorta ratio as a method for quantifying volume depletion in dogs. In 12 normal beagle dogs, furosemide was administered intravenously at a dose of 1 mg/kg, every 2 h, for 8 h, to induce consecutive volume depletion. Every 30 min after administration, ultrasonographic images of the caudal vena cava and aorta, and physical and biological parameters related to dehydration were acquired. On transverse and longitudinal planes of caudal vena cava and aorta images, the height and area of the caudal vena cava and aorta were measured to calculate the caudal vena cava/aorta ratios. All images were acquired by approaching from the right intercostal space with the dogs in left lateral recumbency. A negative correlation was present between the percentage of weight loss in dogs and all four investigated caudal vena cava/aorta ratios (transverse plane width of the caudal vena cava [TW]/aorta; transverse plane height of caudal vena cava [TH]/aorta; longitudinal plane area of the caudal vena cava [TA]/aorta; and longitudinal plane maximal height of the caudal vena cava [L]/aorta). Significant differences (P < 0.001) were seen between dogs with and without clinical signs of dehydration for all caudal vena cava/aorta ratios. Findings indicated that ultrasonographic caudal vena cava/aorta ratios are feasible methods for quantifying volume depletion and for use as an adjunct to standard subjective methods for estimating hydration status in dogs.
The primary determinant of influenza virus infectivity is the type of linkage between sialic acid and oligosaccharides on the host cells. Hemagglutinin of avian influenza viruses preferentially binds to sialic acids linked to galactose by an α-2,3 linkage whereas hemagglutinin of human influenza viruses binds to sialic acids with an α-2,6 linkage. The distribution patterns of influenza receptors in the avian respiratory tracts are of particular interest because these are important for initial viral attachment, replication, and transmission to other species. In this study, we examined the distribution patterns of influenza receptors in the respiratory tract of chickens, ducks, pheasants, and quails because these species have been known to act as intermediate hosts in interspecies transmission. Lectin histochemistry was performed to detect receptor-bearing cells. Cell-specific distribution of the receptors was determined and expression densities were compared. We observed species-, site-, and cell-specific variations in receptor expression. In general, receptor expression was the highest in quails and lowest in ducks. Pheasants and quails had abundant expression of both types of receptors throughout the respiratory tract. These results indicate that pheasants and quails may play important roles as intermediate hosts for the generation of influenza viruses with pandemic potential.
The renal cortical thickness (RCT) reflects the pathological condition of the kidney, and measuring this parameter can help diagnose renal fibrosis in dogs. The normal reference range of RCT in dogs is broad (3-8 mm) because of the extreme diversity in body size. Therefore, this retrospective, reference interval, and observational design study aimed to establish a normal reference range for RCT in dogs measured using ultrasound by considering bodyweight (BW), body surface area (BSA), body condition score (BCS), and abdominal aorta (Ao) diameter. A total of 60 dogs met the inclusion criteria, and abdominal ultrasound images and medical records of these dogs were analyzed. RCT was measured at 1-3 points where the renal capsule and broad base of the medullary pyramid were clearly observed. Ao diameter was measured caudal to the branch of the left renal artery in the mid-sagittal view. The RCT showed positive correlations with BW and BSA and a negative correlation with BCS, which can be described as follows: RCT (mm) = 0.131 × BW − 0.166 × BCS + 3.580. The RCT:Ao ratio was 0.70 ± 0.09 (mean ± standard deviation). No significant differences were found in the RCT:Ao ratio depending on BW or BCS. In conclusion, the RCT:Ao ratio and relative RCT considering both BW and BCS are potentially useful for evaluating the normality of the renal cortex on ultrasound examination in dogs with various physiques.
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