Brucella Coombs gel test can be used as a screening test with high sensitivity. By the help of pink Brucella antigen precipitation, the tests' evaluation is simple and objective. In addition, determination of Brucella antibody by rapid titration offers another important advantage.
Objective:
The studies on children with COVID-19 are very limited. The aim of this study is to reveal the effect of serum 25-hydroxy vitamin D level on clinical and laboratory parameters.
Materials and Methods:
The study included 74 children (35 boys and 39 girls) diagnosed with COVID-19. The retrospective data were obtained from the file records of the patients. Seventy-four patients were divided into 3 groups (group 1, deficient; group 2, insufficient; and group 3, sufficient) according to their serum 25-hydroxy vitamin D levels.
Results:
The mean age of all patients was 113.25 ± 64.55 months. The mean leucocyte count was substantially higher in group 3 compared to groups 1 and 2 (
P
= .05 and
P
= .002, respectively). The mean lymphocyte and platelet count in group 3 was remarkably higher than both groups 1 and 2 (
P
= .001 and
P
= .002; and
P
= .04 and
P
= .01, respectively). The mean serum parathyroid hormone concentration in group 1 was markedly higher than both groups 2 and 3 (
P
= .003 and
P
= .002, respectively) while the mean serum 25-hydroxy vitamin D level in group 1 was remarkably lower than both groups 2 and 3 (
P
= .001 and
P
= .001, respectively). Serum 25-hydroxy vitamin D concentrations were positively correlated with leucocyte, lymphocyte, and platelet counts (
r
= 0.221,
P
= .05;
r
= 0.396,
P
= .001; and
r
= 0.249,
P
= .03, respectively) while there was a negative correlation with parathyroid hormone concentrations (
r
= −0,436,
P
= .001).
Conclusion:
This study suggests that COVID-19 has a benign course in children and that serum 25-hydroxy vitamin D concentration may have a role in the lymphocyte count.
Objective: This study was planned to detect methicillin resistance in Staphylococcus aureus strains by conventional phenotypic methods and polymerase chain reaction for mecA gene and to compare the results of these tests for their sensitivity and specificity. Methods: Eighty six methicillin-resistant S. aureus (MRSA) and 30 methicillin-sensitive S. aureus (MSSA) strains from various clinical samples were included in this study. Methicillin resistance was detected by oxacillin and cefoxitin disk diffusion method. Presence of colonies on MRSA chromogenic agar was looked for methicillin resistance. The presence of the mecA gene investigated by polymerase chain reaction is considered to be the gold standard test, and sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated accordingly.
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