In biosensors research, much effort has been made to achieve high sensitivity to detect lower concentrations of analyte in a solution by testing different kinds of materials. In this paper, we present a biosensor based on Bloch surface waves made of photonic crystal (PhC) including graphene nanolayers under the Kretschmann configuration. The band structures, surface modes, reflectivity, and sensitivity of the PhC biosensor are calculated by the transfer matrix method and results are compared with those of the structure without graphene layers. Our investigations show that the angular sensitivity of the biosensor considerably increases in the presence of the graphene layers. Moreover, we study the effect of the number of the graphene layers placed on the surface of the biosensor on the performance of our proposed biosensor. The results reveal that the sensitivity of the biosensor is enhanced by increasing the number of graphene layers on the surface due to the π-stacking interactions between graphene's honeycomb cells and the carbon rings in biomolecules. Furthermore, our results show that the phase sensitivity is higher than the angular sensitivity, which can promote the accuracy of the calculations.

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