Genetic variation in UDP-glucuronosyltransferase 1A1 gene (UGT1A1) is a lithogenic risk factor for gallstone formation. This study aimed to assess genotype and allele frequencies of common UGT1A1 variants in patients with gallstone and hepatitis B virus (HBV)-related hepatic failure. This study enrolled 113 healthy individuals (CTRL), 54 patients with HBV infection (HBV), 134 patients with gallstone-free hepatic failure and HBV infection, and 34 patients with gallstone-related hepatic failure and HBV infection (GRHF). Peripheral venous blood samples were collected for genomic DNA isolation. Polymerase chain reaction amplification was carried out for UGT1A1, followed by direct sequencing. Analysis for genotype and allele frequencies of UGT1A1 variants (UGT1A1*6, UGT1A1*27, UGT1A1*28, and UGT1A1*60) was performed. The allele distributions of the four groups did not deviate from Hardy–Weinberg equilibrium. Allele (A) and genotype (CA) frequency distributions of UGT1A1*27 were significantly different between GRHF and CTRL, or between GRHF and HBV. GRHF and CTRL exhibited significant differences in allele (A) and genotype (CA) frequency distributions of UGT1A1*28. Linkage disequilibrium analysis suggested that haplotype G-G-[TA]7-T may be associated with gallstone in HBV-related hepatic failure. Our data reveal that UGT1A1*27 and UGT1A1*28 variants are significantly observed in patients with GRHF compared to healthy individuals.
Objective: Cardiovascular disease is a series of diseases with high morbidity, high fatality rate, rapid changes in condition, and it is highly prone to emergencies and severe illnesses, and it is the primary disease that leads to death of patients. The modified early warning score (MEWS) is lacks specialized indicators and can't personalize risk prediction for cardiovascular patients. Explore the establishment of a scoring system suitable for evaluating the condition of inpatients in the Department of Cardiovascular Medicine to provide a reference basis for accurate assessment and adequate early warning of the patient's disease, and for targeted nursing plans. Methods: The questionnaire was designed through literature analysis, brainstorming, and expert interviews, and a questionnaire survey was conducted for nursing staff in the Department of Cardiology. Analyze the survey results through SSPS software. Results: According to the survey analysis, 73.9% of the experts surveyed considered it necessary or very necessary to establish a modified early warning score in cardiovascular medicine (MEWS); Approx. Chi-square value of the Bartlett sphericity test was 3760.769 (with 28 degrees of freedom), which reached a significant level (P< 0.000), indicating that the reliability and validity of the scale were high. The corrected MEWS score scale was composed of 8 observation indexes, including systolic blood pressure, diastolic blood pressure, respiration, heart rate, oxygen saturation, arrhythmia, consciousness, and chest pain. Conclusion: Cardiovascular medical nursing staff had a high degree of approval for establishing the corrected MEWS score; The constructed MEWS score for cardiovascular internal disease correction is feasible, objective and practical to a certain extent, and can provide a basis for further exploration of practical application.
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