The occurrence of osteoarthritis (OA) is highly associated with the reduced lubrication property of the joint, where a progressive and irreversible damage of the articular cartilage and consecutive inflammatory response dominate the mechanism. In this study, bioinspired by the super-lubrication property of cartilage and catecholamine chemistry of mussel, we successfully developed injectable hydrogel microspheres with enhanced lubrication and controllable drug release for OA treatment. Particularly, the lubricating microspheres (GelMA@DMA-MPC) were fabricated by dip coating a self-adhesive polymer (DMA-MPC, synthesized by free radical copolymerization) on superficial surface of photo-crosslinked methacrylate gelatin hydrogel microspheres (GelMA, prepared via microfluidic technology), and encapsulated with an anti-inflammatory drug of diclofenac sodium (DS) to achieve the dual-functional performance. The tribological test and drug release test showed the enhanced lubrication and sustained drug release of the GelMA@DMA-MPC microspheres. In addition, the functionalized microspheres were intra-articularly injected into the rat knee joint with an OA model, and the biological tests including qRT-PCR, immunofluorescence staining assay, X-ray radiography and histological staining assay all revealed that the biocompatible microspheres provided significant therapeutic effect against the development of OA. In summary, the injectable hydrogel microspheres developed herein greatly improved lubrication and achieved sustained local drug release, therefore representing a facile and promising technique for the treatment of OA.
Osteoarthritis
(OA) is a chronic joint disease and its progression
and pathogenesis are highly associated with the significant increase
of joint friction and overproduction of reactive oxygen species (ROS)
in inflammation. Combination of ROS elimination and lubrication enhancement
may provide a novel strategy for the treatment of OA. In the present
study, a pure biomaterial and nondrug system P(DMA-co-MPC), synthesized via free radical copolymerization, was designed
and developed for the first time using 2-methacryloxyethyl phosphorylcholine
(MPC) as a bioinspired lubricant and N-(3,4-dihydroxyphenethyl)methacrylamide
(DMA) as an ROS scavenger. Our results showed that the P(DMA-co-MPC) aggregates could efficiently eliminate the ROS radicals
and provide good lubrication property by adjusting the molar ratio
of DMA and MPC in the copolymer. It is attributed to the antioxidant
function of the hydroquinone moiety in DMA and the hydration lubrication
effect of the zwitterionic phosphocholine group in MPC. Furthermore,
the in vitro experiments demonstrated that the P(DMA-co-MPC) showed good biocompatibility with MC3T3-E1 cells and intracellular
anti-inflammatory property by inhibiting the production of ROS and
regulating the expression levels of pro-inflammatory cytokines, pain-related
gene, anabolic genes, and catabolic genes. In conclusion, the drug-free
P(DMA-co-MPC) aggregates developed herein can achieve
dual functions of lubrication enhancement and anti-inflammatory effect
and thus they may be representative as promising candidates for the
treatment of OA.
Visible light-responsive dual-functional biodegradable mesoporous silica nanoparticles with drug delivery and lubrication enhancement were constructed by supramolecular interaction between azobenzene-modified mesoporous silica nanoparticles (bMSNs-AZO) and β-cyclodextrin-modified poly(2-methacryloyloxyethyl phosphorylcholine) (CD-PMPC). Visible...
Osteoarthritis is a chronic inflammatory disease characterized by cartilage degeneration. Anti-inflammatory therapy has limited effects, and effective reduction of cartilage wear through lubrication is essential. In vivo stability and lubricity of commercial lubricants are insufficient, leading to failure of lubricating treatment and progression of osteoarthritis. To address these issues, new therapeutic methods combining anti-inflammation and stable highperformance lubrication are developed. Inspired by the sliding mechanism of snowboards, a "nano-snowboard" by modifying molybdenum disulfide (MoS 2 ) with a biomimetic phospholipid polymer poly (dopamine methacrylamide-co-2-methacryloyloxyethyl phosphorylcholine) (PDMPC) and loading anti-inflammatory drug diclofenac sodium (DS), namely MoS 2 -PDMPC-DS, has been synthesized. MoS 2 with a 2D layered structure and photothermal properties, serves as solid lubricant and drug carrier. Meanwhile, the modification of PDMPC on the surface of MOS 2 avoids the oxidative denaturation of MoS 2 in a physiological environment, forming solid-liquid composite lubrication and improving the lubricity and stability of MoS 2 in the joint cavity. In vitro and in vivo experiments show that MoS 2 -PDMPC-DS can stay in the joint cavity for more than one week and exert long-lasting lubrication and anti-inflammatory effects to treat osteoarthritis effectively. This solid-liquid composite lubricating nano-snowboard provides a new idea for synergistic anti-inflammatory and lubricating treatment of osteoarthritis.
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