Summary My cobacterium /eprae was isolated fr om skin-punch biopsies of 2 untreated lepromatous leprosy patients. The bacteria were enumerated, diluted lO-fold and cultured in Middlebrook 7H9 medium supplemented with albumin, dextrose, catalase and 14 C-palmitic acid. The cultures were incubated at 33°C in a modified Buddemeyer radiorespiratory detection vessel. Those cultures containing at least 10 7 mycobacteria demonstrated a progressive evolution of 14 C02 .
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