With the development of Internet technology and communication technology, high quality Internet video has become an important dissemination of media information, and also become an Internet service used by more and more users. Analysis of online video user behavior will have great significance for the development of online video platform and user monitoring public opinion. Big Data as a new solution for data processing, to be able to do a wide range of mass data processing and mining. This paper combines with the Big Data technology and systematic expounds data acquisition technology for mass data related to network video. And a brief overview of the principle of Hadoop platform for data analysis.
After studying the relationship between the moisture content of crops and their relative permittivity, the principle of capacitive sensor and the research results of measuring micro-capacitance, this paper summarizes the theoretical basis of dielectric properties of grain, the dielectric properties of wheat, corn and rice, the relationship between the dielectric properties of these crops and their water content. With these theory analyses, the paper gives a full look on the grain moisture detection system by the means of dielectric properties, which consists of six parts, a cylindrical capacitor, signal conditioning circuits, single-chip control module with an A/D converter, temperature detection module, keyboard module and display module. The system is simple, and can adapt to extreme environment and accomplish the rapid detection in manufacturing. A description is given of the principle, its hardware circuit and software programming flow chart.
Bone morphogenetic proteins (BMPs) are cytokines from the TGF-β superfamily, with important roles during embryonic development and in the induction of bone and cartilage tissue differentiation in the adult body. In this contribution, We report here the application of small ubiquitin-related modifier (SUMO) fusion technology to the expression and purification of human BMP-14. The fusion protein expressed in a soluble form was purified to a purity of 90% by Ni-IDA chromatography. After the SUMO-BMP14 fusion protein was cleaved by the SUMO protease at 30 °C for 1 h, the cleaved sample was re-applied to a Ni-IDA. Finally, about 45 mg recombinant hBMP-14 was obtained from 1 litre bacterial culture with no less than 95% purity. The purified hBMP-14 dimer was over 90% purity and could induce the expression of alkaline phosphatase activity in C2C12 cells in a dose-dependent manner. Thus the SUMO-mediated peptide expression and purification system potentially could be employed for the production of other homodimeric proteins.
Micro-video label classification system (MLCS) is designed to tag and classify micro-videos by a specific set of labels, which are extracted from users’ comments on the internet. These labels not only describe the micro-video’s features in various aspects, but also deliver the audiences’ attitudes and concern. Combining the high-ranking labels can effectively organize the creative elements to help producing new video scripts and categories. It breaks the traditional limit of linear producing process and makes the micro-videos’ story more topical and integrate.
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