Single-mode field has advantages in active detection, underwater communication, bottom backscattering measurement, etc. Some of papers have reported on the theory and simulation about the single-mode excitation by open- and closed-loop methods, respectively. The experiment in the tank has also been reported many years ago, but the experiment in the water has not been reported yet. In this paper, an effective closed-loop method for exciting the single-mode field is discussed, which can obtain the optimum estimation of the matrix of the Green’s function in the shortest feedback times, with high efficiency of emission of the single-mode signal. The experiments at the different sites in different seasons are reported. The vertical source array with the center frequency at 700 Hz was used as the source in the experiment. The experimental results show that when the source array spans almost the effective water depth the single-mode field with high quality can be excited. The first three single modes were excited at the sites with the water depth of 14 m and 36 m respectively. The experimental results show that more than 97% of the energy of the received field is concentrated in the single-mode.
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