Highly sensitive splitless programmed temperature vaporizing (PTV)-large volume injection (LVI)-GC-MS-negative chemical ionization (NCI) method was developed and validated for the trace detection of explosives and related compounds from environmental matrices.
A novel analytical technique for the structural elucidation of compounds bearing a tertiary amine side chain via "in vial" instantaneous oxidation and liquid chromatography mass spectrometry (LC-MS) was developed. A series of lidocaine homologs and benzimidazole derivatives with a major/single amine representative base peak in both their EI-MS and ESI-MS/MS spectra were subjected to oxidation by a 0.1% solution of hydrogen peroxide (including several O/ O exchange experiments), followed by LC-ESI-MS/MS analysis. The N-oxide counterparts promoted extensive fragmentation with complete coverage of all parts of the molecule, enabling detailed structural elucidation and unambiguous identification of the unoxidized analytes at low nanogram per milliliter levels.
Fluorine atoms play an important role in all branches of chemistry and accordingly, it is very important to study their unique and varied effects systematically, in particular, the structure-physicochemical properties relationship. The present study describes exceptional physicochemical effects resulting from a H/F exchange at the methylene bridge of gem-difunctional compounds. The Δlog P (CF2-CH2) values, that is, the change in lipophilicity, observed for the CH 2 /CF 2 replacement in various α,α-phenoxyand thiophenoxy-esters/amides, diketones, benzodioxoles and more, fall in the range of 0.6-1.4 units, which for most cases, is far above the values expected for such a replacement. Moreover, for compounds holding more than one such gem-difunctional moiety, the effect is nearly additive, so one can switch from a hydrophilic compound to a lipophilic one in a limited number of H/F exchanges. DFT studies of some of these systems revealed that polarity, conformational preference as well as charge distributions are strongly affected by such hydrogen to fluorine atom substitution. The pronounced effects described, are a result of the interplay between changes in polarity, H-bond basicity and molecular volume, which were obtained with a very low 'cost' in terms of molecular weight or steric effects and may have a great potential for implementation in various fields of chemical sciences.
A new analytical technique for the structural elucidation of four representative phenidate analogues possessing a secondary amine residue, which leads to a major/single amine-representative fragment/product ion at m/z 84 both in their GC-EI-MS and LC-ESI-MS/MS spectra, making their identification ambiguous, was developed. The method is based on "in vial" chemical derivatization with isobutyl chloroformate in both aqueous and organic solutions, followed by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS/MS). The resulting carbamate derivatives promote rich fragmentation patterns with full coverage of all substructures of the molecule, enabling detailed structural elucidation and unambiguous identification of the original compounds at low ng/mL levels.
The human respiratory system is a highly complex matrix that exhales many volatile organic compounds (VOCs). Breath‐exhaled VOCs are often “unknowns” and possess low concentrations, which make their analysis, peak digging and data processing challenging. We report a new methodology, applied in a proof‐of‐concept experiment, for the detection of VOCs in breath. For this purpose, we developed and compared four complementary analysis methods based on solid‐phase microextraction and thermal desorption (TD) tubes with two GC–mass spectrometer (MS) methods. Using eight model compounds, we obtained an LOD range of 0.02–20 ng/ml. We found that in breath analysis, sampling the exhausted air from Tedlar bags is better when TD tubes are used, not only because of the preconcentration but also due to the stability of analytes in the TD tubes. Data processing (peak picking) was based on two data retrieval approaches with an in‐house script written for comparison and differentiation between two populations: sick and healthy. We found it best to use “raw” AMDIS deconvolution data (.ELU) rather than its NIST (.FIN) identification data for comparison between samples. A successful demonstration of this method was conducted in a pilot study (n = 21) that took place in a closed hospital ward (Covid‐19 ward) with the discovery of four potential markers. These preliminary findings, at the molecular level, demonstrate the capabilities of our method and can be applied in larger and more comprehensive experiments in the omics world.
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