The hybrid Silver(Ag) nanoparticles were synthesized by gamma-irradiation, and characterized by field-emission transmittance electron microscopy (FE-TEM), field-emission scanning electron microscopy (FE-SEM), and energy dispersive X-ray spectroscopy (EDXS). The electron microscopy images show that well-dispersed Ag nanoparticles of about 3-7 nm were loaded to the outer and inner side of SiO2 nanoparticles of about 5-30 nm. Antifungal efficiency of the hybrid nanoparticles was tested against Aspergillus flavus ATCC 64025, Aspergillus fumigatus ATCC 16424, Aspergillus terreus ATCC 46941, Candida albicans ATCC 10231, Candida albicans A207(clinical isolate), Candida glabrata ATCC 48435, Candida krusei ATCC 6258, Candida lusitaniae ATCC 42720, Candida parapsilosis ATCC 34136, Candida tropicalis ATCC 13803, Cryptococcus neoformans ATCC 36556, and Mucor ramosissmus ATCC 90286. At the presence of hybrid Ag nanoparticles at 1 ppm, they did not grow fully even after 58 hrs except for Cryptococcus neoformans ATCC 36556. The hybrid Ag nanoparticle can be used for antifungal agents.
In order to prepare the enzyme electrode for sensing of chemical molecules, the Au was coated on the surface of PP film by sputter coater. Polyaniline (PANI) was polymerized on the surface of the coated Au film by electrochemical polymerization of aniline in order to use working electrode. Subsequently, horseradish peroxide (HRP) was immobilized on PANI electrode as follows: Method I. The HRP was immobilized on the surface of PANI electrode by using Au nanoparticle as linker (physical adsorption). Method II. The HRP was immobilized on the surface of PANI electrode by using 2-aminothiophenol and Au nanoparticle as linker (Self-assembling immobilization). Method III. HRP was directly immobilized with PANI electrode by using glutaric dialdehyde as linker (covalent bonding). The sensing efficiency of the prepared HRP-PANI electrode was also examined. The sensing efficiency of the HRP-PANI electrode for H2O2 was as following order; Method I > Method III > Method II..
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