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CYP17 gene is the key in the metabolic pathway of sexual steroids, it codes for two enzymes 17–alpha hydroxylase and 17, 20 lyase who play an important role in the production of sex steroid hormones. The current study included study of the relationship of this gene with PCOS women in reproductive age. Blood samples were collected from two groups of women: first group consists of 98 women infected with polycystic ovaries syndrome, the second consists of 25 healthy women to detect the presence of mutation within the gene, then calculate allele frequency of mutant and wild allele for this gene, then repeat genotypes in infected women and compared with healthy once, were calculated body mass index BMI of two groups and measure the level of sex hormones (LH, FSH, Prolactin, Testosterone) in both groups, were the results showed significant differences between patients and healthy women in BMI and the level of hormones (LH, Prolactine) while there is no significant differences in the (FSH, Testosterone) hormone. Three pattern of genotype were obtained: TT, homozygous wild, (TC) much lower than the frequency in the healthy 0.82 while the frequency of allele C among patients 0.44, which is more than double than in healthy 0.18. The current results showed absence CC genotype in healthy women and appeared only in women with PCOS. This monitor the presence of link between this gene and PCOS as may be considered the mutation -34 (T C) in the promoter of gene CYP17 marker of the disease.

Peroxisome Proliferator Activated Receptors Gamma (PPAR-ᵞ) is a nuclear transcription factor, which Protein receptors for insulin and have a significant role in insulin resistance and Type 2 Diabetes mellitus in addition to the role of the gene encoded in the differentiation Adipocyte and the development of Adipose tissue. at present research has been detected substitution silent mutation C T in  the exon 6 of the gene PPAR-ᵧ in 111 women, including 73 obese women with Polycystic ovarian syndrome and 38 healthy women . in addition we determined certain biochemical variables  (Blood Sugar, HDL-C, Triglycerides) and find a body mass index (BMI) and compared between the two groups of women where the results showed significant difference value of BMI and the concentration of each of the (Blood Sugar, HDL-C, Triglycerides) and at (0.05 and 0.01) among Patient women and Healthy the results showed significant difference in mutant T allele frequency between them . The value in Obese PCOS women was (0.336) compared to the value at the healthy women (0:29) this indicates the a correlation between substitution silent mutation C T in exon 6 for PPAR-ᵧ and disease

Inhibin-B is produced by sertoli cells and acts as a negative feedback mechanism of follicular stimulation hormone (FSH). In order to evaluate the effect of Inhibin-B in men with Azoospermia, the study measured the concentrations of the following hormones: Inhibin-B, FSH, Luteinizing Hormone Prolactin (PRL), (LH) and Testosterone (Testo). Serum for 30 normal men semen and 50 men with Azoespermia (zero sperm count in sperm). The mean concentration of the hormone Inhibin-B in patients with Azoospermia was (135.7 ±87.6) pg/ml, while in normal men (175.6 ±47.9) Pg / ml with a significant value of the group of healthy patients at a significant level (p≤0.01). The mean serum FSH concentration in patients was (13.7 ± 12.27) lU / L whereas in normal men (4.1 ± 2.84) lU / L, the relationship was significant between patients and healthy at a significant level (P≤0.01) the correlation with the Inhibin-B  hormone is reversible relationship. The mean concentration of LH in patients was (12.8±11.87) IU/L and in the healthy (7.9±5.57) IU/L and the relationship between them was significant  (P≤0.05) and the correlation with the Inhibin-B hormone is positive relationship. The mean concentration of the  Testosterone In the patients was  (2.37 ± 1.22) IU / L and in the healthy (5.1 ± 1.96) IU/ L, the relationship was of significant value at P≤0.01 and the correlation with the Inhibin-B hormone is positive relationship, and there is a difference in the mean concentration of PRL between the patients and the healthy where it reaches (8.88 ±5.16) IU / L in patients and (8.03 ± 4.2) IU / L in the healthy. where the relationship was non-significant value between both patients and healthy and the correlation with the Inhibin-B hormone is positive relationship

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