The alpha (1) fucosyltransferase gene (FUT1) is a candidate gene for controlling the adhesion of Escherichia coli F18 receptor. Indeed, a single-nucleotide polymorphism, c.307G>A, located in the gene is such that pigs with AA genotype are resistant to entero-toxigenic E. coli F18, whereas those with AG and GG genotypes are sensitive. An experiment was carried out in northern Vietnam from March 2016 to May 2017 to determine FUT1 genotype frequencies and the effect of these genotypes on production performance of Yorkshire pigs. A total of 613 animals were genotyped using polymerase chain reaction – restriction fragment length polymorphism method. The body weights at birth, weaning, initial fattening period, and final fattening period were collected from 611, 516, 479, and 418 animals, respectively, whereas backfat thickness, depth of longissimus dorsi, and lean meat percentage were recorded from 328 animals. The frequencies of FUT1 genotypes were found to be in Hardy–Weinberg equilibrium (P = 0.51). Effect of FUT1 genotype was not observed for all production traits (P > 0.05), whereas final body weight and depth of longissimus dorsi were significantly different between females and males (P < 0.05). These results suggest that selection of Yorkshire pigs resistant to entero-toxigenic E. coli F18 could be effective without adversely affecting average daily gain and lean meat.
The objective of the present study is to investigate the reproduction, production and semen traits of the stressnegative Piétrain line of pigs in the north of Vietnam, as well as to identify various factors (halothane genotype, generationorigin, parity, sex and month of the year) acting on these traits. The experiment was carried out on a purebred nucleus on Dong Hiep farm, Haiphong province in Vietnam. The reproduction performances and the sperm quality measures were based on 12 sows and 5 boars imported from Belgium as well as on 29 sows and 14 boars born in Vietnam, while the production performances were recorded only from 251 animals born in Vietnam. No significant differences were observed between halothane genotypes of sows for reproduction traits (P > 0.0578). At the age of 7.5 months, bodyweight, backfat thickness and longissimus depth (7.5) of homozygous-dominant pigs were higher (P < 0.05) than in heterozygotes. Homozygous-dominant boars had higher total number of spermatozoa than heterozygote boars (P < 0.05); similarly, Vietnam boars also presented higher total number of spermatozoa values than Belgium boars (P < 0.001). Vietnam sows had longer duration of pregnancy, more piglets born alive and heavier litter weight of weaning than Belgium sows (P < 0.01). Bodyweight at birth, at weaning, at 2, 5.5 and 7.5 months of age increased from the first to third parity (P < 0.05). Gilts had more lean meat than intact boars (P < 0.01). Compared with observations made in temperate countries, the reproduction and production performances, sperm concentration and total number of spermatozoa of stress-negative Piétrain pigs were reduced, whereas lean meat percentage was not affected. This might be due to the different climate, although improving values for animals born in Vietnam might indicate adaptation of the animals and/or of the technicians with that breed. The results support the interest of the use of this new Piétrain line in tropical countries with ecological and climatic conditions similar to Vietnam.
IntroductionDiagnostic test evaluation for African swine fever (ASF) in field settings like Vietnam is critical to understanding test application in intended populations for surveillance and control strategies. Bayesian latent class analysis (BLCA) uses the results of multiple imperfect tests applied to an individual of unknown disease status to estimate the diagnostic sensitivity and specificity of each test, forgoing the need for a reference test.MethodsHere, we estimated and compared the diagnostic sensitivity and specificity of a novel indirect ELISA (iELISA) for ASF virus p30 antibody (Innoceleris LLC.) and the VetAlert™ ASF virus DNA Test Kit (qPCR, Tetracore Inc.) in field samples from Vietnam by assuming that disease status 1) is known and 2) is unknown using a BLCA model. In this cross-sectional study, 398 paired, individual swine serum/oral fluid (OF) samples were collected from 30 acutely ASF-affected farms, 37 chronically ASF-affected farms, and 20 ASF-unaffected farms in Vietnam. Samples were tested using both diagnostic assays. Diagnostic sensitivity was calculated assuming samples from ASF-affected farms were true positives and diagnostic sensitivity by assuming samples from unaffected farms were true negatives. ROC curves were plotted and AUC calculated for each test/sample combination. For comparison, a conditionally dependent, four test/sample combination, three population BLCA model was fit.ResultsWhen considering all assumed ASF-affected samples, qPCR sensitivity was higher for serum (65.2%, 95% Confidence Interval [CI] 58.1–71.8) and OF (52%, 95%CI 44.8–59.2) compared to the iELISA (serum: 42.9%, 95%CI 35.9–50.1; OF: 33.3%, 95%CI 26.8–40.4). qPCR-serum had the highest AUC (0.895, 95%CI 0.863–0.928). BLCA estimates were nearly identical to those obtained when assuming disease status and were robust to changes in priors. qPCR sensitivity was considerably higher than ELISA in the acutely-affected population, while ELISA sensitivity was higher in the chronically-affected population. Specificity was nearly perfect for all test/sample types.DiscussionThe effect of disease chronicity on sensitivity and specificity could not be well characterized here due to limited data, but future studies should aim to elucidate these trends to understand the best use of virus and antibody detection methods for ASF. Results presented here will help the design of surveillance and control strategies in Vietnam and other countries affected by ASF.
Porcine mucin 4 (MUC4) is a candidate gene for controlling the adhesion of the enterotoxigenic Escherichia coli (ETEC) F4 receptor. Polymorphisms of the MUC4 gene have been used as markers to identify the susceptibility of neonatal diarrhea in piglets for breeding selection. The objective of this study was to evaluate the effects of MUC4 g.243A>G polymorphisms on the production traits of Landrace and Yorkshire pigs in Vietnam. A total of 1,057 Landrace and 1,361 Yorkshire piglets were used to estimate the allelic and genotypic frequencies of the polymorphisms. Body weights at birth, at weaning, at initial fattening (IBW), and at the end of fattening period (FBW), backfat thickness (BFT), and depth of the longissimus dorsi muscle (DLD) were measured and lean meat percentage was estimated. Frequency of the susceptibility allele A to ETEC was higher than the resistance allele G for both breeds based on genotyping piglet tails collected at birth. The AA, AG, and GG genotypes were present in Yorkshire while GG was not found in Landrace. The production traits were not affected (P >0.05) by MUC4 polymorphisms except BFT and DLD (P <0.05). There were interactions between gender and MUC4 genotype (P <0.05) for IBW, FBW, average daily gain, and DLD. These traits of GG males were significantly higher than those of GG females (P <0.05). The results suggest that selecting pigs carrying the GG genotype of MUC4, known as providing resistance to ETEC, do not negatively affect productive performance in Landrace and Yorkshire pigs.
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