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The relationship between the dynamic rheological properties of heatinduced gels of actomyosin (natural actomyosin) and the denaturation of actin in actomyosin during storage without ATP at pH 6.0 and 0°C was investigated using biochemical and dynamic rheological measurements. The complex modulus of gels after heating actomyosin containing 0.5 or 1.5 M KCl (pH 6.0) at 80°C increased with increasing storage time. The dynamic rheological behaviour during heat gelation of actomyosin in 1.5 M KCI indicated that the first rheological transition peak in the 50-53°C range induced by the presence of F-actin gradually disappeared with increasing storage time. However, in 0.5 M KCI, this transition peak clearly remained even after 15 days. The time course of denaturation of actin in actomyosin treated with 1.5 M KCI at pH 6.0 showed an increase in the percent denaturation after the storage was started, and about 100% of the actin became denatured after 21 days. In the case of 0.5 M KCI, unlike 1.5 M, the denaturation of actin occurred quickly within the first 5 days and then did not proceed. A sigmoidal relationship was found between the percent denaturation of actin and the KCl concentration added, the greatest change occurring at KCI concentrations between 0.5 and 1.0 M. The data indicated that the change in the property of actin in actomyosin during storage at low temperature exerts a great influence on the viscoelasticity of heat-induced gels of actomyosin.

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Somatosensory evoked response (SER) was studied in 70 patients with neurosurgical disorders and 20 nomal, healthy men, in order to find out whether SER is helpful as a diagnostic aid for neurological diseases. Electric stimulations were given to the tibial nerve to provoke monosynaptic reflex of the M. soleus. The strength ranges of electric stimulations were classified as follows: (1) The weak range; H-wave of the monosynaptic reflex can be seen alone. (2) The medium range; both of Hand Mwave are appeared. (3) The strong range; H-wave is decreased in amplitude, or Mwave can be seen alone. SERs were led from the scalp electrodes placed at the vertex (CZ), left (C3) and right (C4) parietal regions by means of the averaging computer (ATAC-501). In the normal subjects, the response appeared to have a least 8 distinct peaks, beginning with a small triphasic wave (ABC) with the latency of 37, 57 and 75 msec, respectively, followed by a negative peak (D) with that of 110 msec and subsequent