Context
Michelia champaca
L. (Magnoliaceae) has been known since ancient times for its rich medicinal properties.
Objective
The ethanol extract of
Michelia champaca
leaves (EEMC) was evaluated on depression and anxiety using
in vivo
and
in silico
studies
Materials and methods
Swiss albino mice were divided into control, standard, 100 and 200 mg/kg b.w. EEMC groups and for drug administration using oral gavage. The antidepressant activity was evaluated using forced swim test (FST) and tail suspension test (TST) whereas the anxiolytic activity through elevated plus maze and light and dark tests. The
in silico
studies included molecular docking against human potassium channel KCSA-FAB and human serotonin transporter, and ADME/T analysis.
Results
Open arm duration and entries were comparable between 200 mg/kg b.w. group (184.45 ± 1.00 s and 6.25 ± 1.11, respectively) and that of diazepam treated group (180.02 s ± 0.40 and 6.10 ± 0.05, respectively). Time spent in the light cubicle was higher (46.86 ± 0.03%), similar to that of diazepam (44.33 ± 0.64%), suggesting its potent anxiolytic activity. A delayed onset of immobility and lowered immobility time was seen at both the treatment doses (FST: 93.7 ± 1.70 and 89.1 ± 0.40 s; TST: 35.05 ± 2.75 and 38.50 ± 4.10 s) and the standard drug imipramine (FST: 72.7 ± 3.72 and TST: 30.01 ± 2.99 s), indicative of its antidepressant ability.
In silico
studies predicted doripenem to induce anxiolytic and antidepressant activity by inhibiting human potassium channel KCSA-FAB and human serotonin transporter proteins, respectively.
Conclusions
EEMC is a rich source of bioactive compounds with strong antidepressant and anxiolytic properties.
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