An experiment was designed to study the in vivo effect of Pasteurella haemolytica A2 infection on the phagocytosis activity of caprine broncho-alveolar macrophages and the extent of pneumonic lesions. Twelve healthy local Kacang goats, about 7 months of age, were divided into two groups of six. Goats in group 1 were inoculated intratracheally with 4 ml inoculum containing 2.8´10 9 colony-forming units (CFU)/ml of Staphylococcus aureus. Goats in group 2 were inoculated intratracheally with 4 ml of inoculum containing 9.5´10 8 CFU/ml of Pasteurella haemolytica A2 isolated earlier from pneumonic lungs of goat. At intervals of 3 and 7 days post-challenge 1 ®ve goats from each group were killed and the lungs were washed with sterile phosphate-buffered saline. Smears were prepared from the lung washing¯uid and the number of macrophages with phagocytic activity was determined. At day 3 post-infection, goats of both groups showed a similar pattern of pneumonic lesion. The lung washing¯uid of goats in group 2 was found to contain numerous neutrophils and macrophages. Goats in group 2 showed signi®cantly (P < 0.05) higher extent of lung lesions than group 1. Similarly, the average extent of lung lesions was signi®cantly (P < 0.05) more severe in group 2 at day 7 post-infection. The lung washing¯uid contained mostly macrophages. The phagocytic activity following S. aureus infection was more ef®cient and signi®cantly (P < 0.01) higher compared with infection by P. haemolytica A2. There were weak correlations between the extent of pneumonic lesion and the phagocytic activity. Thus, goats with poor phagocytic activity were likely to develop more extensive lung lesions.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.