A field experiment was conducted to assess the response of nutrient levels and spacing on seed quality attributes of black gram cv. LBG-625 (Rashmi). Experimental results revealed that fertilizer application of 50:100:100 + Black gram rhizobia (250 g ha-1) + PSB- Bacillus megaterium (250 g ha-1) recorded highest test weight (39.27 g), germination (90.60%), root length (15.77 cm), shoot length (13.43 cm), mean seedling length (29.20 cm), mean seedling dry weight (57.99 mg), seedling vigour index-I (2656), seedling vigour index-II (525), total dehydrogenase activity (0.998), protein content (23.16%), field emergence (86.56 %) lowest electrical conductivity (0.813 dSm-1) were superior over other fertilizer treatments. Among the planting geometry 60 x 10 cm recorded more germination per cent (90.48 %) compared to 45 x 10 cm (87.64 %) and 30 x 10 cm (86.91 %). Interaction of nutrient levels and spacing through the application of 50:100:100 + black gram rhizobia (250 g ha-1) + PSB- B. megaterium (250 g ha-1) with planting geometry 60 x 10 cm recorded more mean seedling length (34.40 cm), mean seedling dry weight (58.30 mg),and field emergence (90.24 %) lowest electrical conductivity (0.776 dSm-1) compared to control. The application of 50:100:100 + Black gram rhizobia (250 g ha-1) + PSB- B. megaterium (250 g ha-1) with planting geometry 60 x 10 cm were considered as seed quality improvement approach in blackgram, therefore conjunctive use of inorganic fertilizers and biofertilizer may be suggested for higher seed quality parameters along with overall betterment of crop.

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The seed coat composition of white (JS 335) and black (Bhatt) soybean (Glycine max (L.) Merr) having different water permeability was studied. Phenols, tannins and proteins were measured, as well as trace elements and metabolites in the seed coats. The seed coat of Bhatt was impermeable and imposed dormancy, while that of JS 335 was permeable and seeds exhibited imbibitional injury. Bhatt seed coats contained comparatively higher concentrations of phenols, tannins, proteins, Fe and Cu than those of JS 335. Metabolites of seed coats of both genotypes contained 164 compounds, among which only 14 were common to both cultivars, while the remaining 79 and 71 compounds were unique to JS 331 and Bhatt, respectively. Phenols are the main compounds responsible for seed coat impermeability and accumulate in palisade cells of Bhatt, providing impermeability and strength to the seed coat. JS 335 had more cracked seed coats, mainly due to their lower tannin content. Alkanes, esters, carboxylic acids and alcohols were common to both genotypes, while cyclic thiocarbamate (1.07%), monoterpene alcohols (1.07%), nitric esters (1.07%), phenoxazine (1.07%) and sulphoxide (1.07%) compounds were unique to the JS 335 seed coat, while aldehydes (2.35%), amides (1.17%), azoles (1.17%) and sugar moieties (1.17%) were unique to Bhatt seed coats. This study provides a platform for isolation and understanding of each identified compound for its function in seed coat permeability.

A field experiment was conducted to know the effect of water regimes on seed quality parameters of rice and the was conducted by using factorial randomized complete block design, with two genotypes (BI-33 and Jaya) and two planting methods (Aerobic and Wetland). The resultants seeds were taken to conduct laboratory experiments pertaining to seed quality attributes and the studies revealed that the BI-33 under aerobic condition had shown a significantly higher seed quality with respect to germination (99%), mean seedling length (26.75cm), seedling dry weight (10.42 mg), SVI-I (2648) and SVI-II (1032) , highest total dehydrogenase activity (0.53 OD @ 480nm), highest amylase activity (14.67 %), highest total soluble protein content (7.15%) and recorded less EC (70.95 μSm-1/ppm). The experimental results revealed that both the genotypes and method of planting contribute for seed quality of the genotypes which were grown under different water regimes. The genotype grown under aerobic condition was better in seed quality parameters compared to wetland condition.