A class of non-coding miRNAs has attracted a lot of interest in the field of forensic sciences. miRNA has a relatively small size thus it is quite stable to the external environmental pressures and factors, which makes it very useful in forensic examination when used as a bio marker. At present, many of the specific miRNAs of body fluids can be distinguished by measuring their level of expression. Fresh blood samples and saliva samples were taken from 3 males and 3 females. RNA was extracted using TRIzol method and cDNA synthesis was done. RT reactions were made using SYBR Green master mix for RT-qPCR for quantification and detection of miRNAs. The designed primers were runs against the sample in qPCR and result was seen and compiled. Two miRNA were detected and expressed in both blood and saliva but they failed to differentiate between blood and saliva due to the non-specific primer binding according the methodology used in this study. Hsa-miR20a and Hsa-miR583 have been found to be non-specific for the blood and saliva. No stark difference could be observed on the basis of which we can say that Hsa-miR20a and HsamiR583 were specific for blood and saliva due to non-specific primer binding. Further research work is required in this domain of body fluids identification and differentiation using miRNA markers.
Bloodstain pattern analysis makes use of the mechanics of bloodstains to estimate the time, nature and the sequence of events at the crime scene. In this study, a quantitative methodology was developed which includes manually calculating the average size of the bloodstains and the synthetic bloodstains to distinguish the impact stain patterns created by different gender and age groups using the two blunt weapons. Impact stains were created by hitting sponges soaked in blood and synthetic blood using a hammer and a cricket bat. A smaller bloodstain size was observed of the group of males aged between 18 to 30 years. Greater amount of spatter and the distribution of the blood spatter were observed with the bat as compared to hammer due to larger surface area of bat. Cast off patterns were studied and were observed to be produced only by the bat which can be attributed to its higher surface area. Results showed difference in the bloodstain diameter produced from human blood and synthetic blood evaluating if the synthetic blood is suitable for use, as well as variation in the average stain size between produced by the four groups using both the fluids.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.