Isolated polymorphonuclear leukocytes induce alterations of the antigen receptor mosaic of autologous red cell surfaces in vitro. Such alterations are represented by decreased expression or loss of MN receptors and the ‘appearance’ of HLe^b- and Le^a-like receptor activities, which can be demonstrated by means of reactions of leukocyteexposed erythrocytes with anti-H lectins and heterologous anti-Le sera and by specific inhibition of these reactions. Since the receptor changes observed were most pronounced on the surfaces of secretar erythrocytes and since demonstrable Lewis-type modifications could not be created on erythrocytes of nonsecretors in these investigations, the antigenic polymorphism unmasked by contact of red cells with polymorphonuclear leukocytes was genetically limited to some extent. These limitations are perhaps due to restricted antigenic variability of the nonsecretor erythrocyte membrane components. The receptormodifying activity is confined to particle fractions of polymorphonuclear leukocytes; it is thermostable up to 70 °C, destroyed at 80 °C, blocked by α(1)-antitrypsin and other natural protease inhibitors, and reaches its peak at physiological pH values between 7.2 and 7.4. These properties correspond with those of the particulate neutral protease of human polymorphonuclear leukocytes. However, glycosidases probably also play a role.
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