L.) plant. A study was initiated through various biostimulant and chemical fertilizer aiming to evaluate effectiveness of arbuscular mycorrhiza fungi (AMF) or seaweed extract and/or humic acid as well as chemical fertilizer (N.P and K) and the combination of them on vegetative growth, seed yield, chemical constituent's, fixed oil productivity and fixed oil composition of chia (Salvia hispanica L.) plant. Plants sprayed by biostimulants gave the significantly highest mean values for all of studied characteristics mentioned above, particularly T6: the combined of mycorrhiza, humic acid, seaweed and recommended dose of NPK followed by T5: recommended dose of NPK (350:200:100 kg/feddan) in the first and second seasons. Meanwhile, T4: seaweed (1 ml/l) ranked the third values in parameters mentioned before in this concern. Whereas, the richest fixed oil percentage was scored by T6, followed descendingly by T5 in the 1 st and 2 nd seasons. Chromatography analysis of chia fixed oil revealed the identification of four components. i.e. palmitic acid, oleic acid, linoleic acid and αlinolenic acid. The main component was α-linolenic acid (43.34 to 53.28%). It can be concluded that T6 was the best for improving growth, seed yield, fixed oil productivity, chemical constituents and fixed oil components of Salvia hispanica L. plant.