Sodium salicylate (NaSal) is a commonly used agent with a wide pharmacological spectrum. The objective of the present study was to investigate the effect of NaSal on anaphylaxis. NaSal (10-1 and 1 mm) significantly inhibited systemic anaphylaxis induced by compound 48/80 in rats. NaSal also significantly inhibited local anaphylaxis activated by anti-dinitrophenyl (DNP) immunoglobulin E (IgE). NaSal (10-1 and 1 mm) significantly inhibited histamine release from rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-DNP IgE. Northern-blot analysis demonstrated that a significantly reduced level of the mRNA of L-histidine decarboxylase was expressed in mast cells treated with NaSal, compared with that without NaSal. NaSal (10-2 and 10-1 mm) had a significant inhibitory effect on anti-DNP IgE-induced tumour necrosis factor-alpha secretion from RPMC. The level of cyclic AMP in RPMC, when NaSal (1 mm) was added, transiently and significantly increased about sixfold compared with that of basal cells. These results suggest a possible use of NaSal in managing mast cell-dependent anaphylaxis.

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IntroductionNitric oxide (NO) regulates blood flow and both muscle contraction and metabolism during exercise. The plasma levels of NO increase after exercise. However, studies that determined the association between the levels of salivary NO and regular exercise are also lacking. The aim of this study was to assess the relationship of the levels of salivary NO with physical activity among Korean elderly.Methods176 subjects aged 48–84 years old were cross-sectionally surveyed. All participants underwent oral and general examination. Age, gender, education level, regular exercise, smoking were evaluated through interview. Periodontal health status was evaluated by clinical attachment loss over 6 mm (CAL6 mm) for six points of 12 index teeth (total 72 sites). The number of CAL6 mm sites and the number of teeth were counted. Fasting plasma glucose level and blood pressure were measured. Unstimulated saliva was collected for 10 min. Salivary NO was determined by the Griess reagent. ANCOVA and Multiple linear regression analyses were applied.ResultsAfter correcting for differences in for age, gender, the number of CAL6 mm sites, the number of teeth, pH in saliva, salivary flow rate, fasting plasma glucose level, systolic blood pressure and diastolic blood pressure, those who exercise everyday had the lowest salivary NO level (66.9 umol/l vs 104.3 umol/l and 121.3 umol/l, p=0.082), which is not significant. Salivary NO levels had a dose-effect relationship with the frequency of regular exercise (β=−13.5, p=0.022).ConclusionThe results of the present study suggest that regular exercise may induce lower levels of NO.