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The joining techniques of lightweight and strong materials in the transport industry (e.g. automotive, aerospace, shipbuilding industries) are very important for the safety of the entire structure. In these industries, when compared with other joining methods, the use of adhesively bonded joints presents unique properties such as greater strength, design flexibility, and reduction in fuel consumption, all thanks to low weight. The aim of this study was the analysis of the tensile fatigue behavior of adhesively bonded glass fiber/epoxy laminated composite single-lap joints with three different specimen types including 30, 40 and 50 mm overlap lengths. In this study, composite adherents were manufactured via vacuum-assisted resin transfer molding and were bonded using Loctite 9461 A&B toughened epoxy adhesive. The effect of a surface treatment method on the bonding strength was considered and it led to an increment of about 40%. A numerical analysis based on a finite element model was performed to predict fatigue life curve, and the predicted results showed good agreement with the experimental investigation.