synopsisAnalysis of polystyrene xttriidards by gel permeation chromatography over B wide range of flow ratev revealed two sources of error in volume measurement. These errors arise from solvent evaporation in the xiphon chamber and from solvent continuing to flow into the siphon during discharge. Appropriate corrections are discussed, and a vapor feedback loop to eliminate the solvent evaporation error is described. The flow rate dependence of the GPC calibration curve, expressed in the corrected elution volumes, a p pears different from that reported in the literature. The corrected flow rate dependence of peak elution volumes is in agreement with the expectation of ditrusion and exclusion theories.
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