One of the targets of modern plant physiology is to identify tools for improving seed germination and plant growth under unfavorable environmental conditions. Seeds of Brassica oleracea rubrum were pretreated with melatonin at concentrations: 1, 10, and 100 microM using a hydropriming method. Air-dried seeds of each experimental variants that were nonpretreated (control), hydroprimed (H) or hydroprimed with melatonin (HM1, HM10, and HM100) were germinated in darkness for 3 days at 25 degrees C. Young seedlings were then transferred to the light and grown for an additional 5 days. Both germination and growth tests were performed in water and in CuSO(4) water solutions in concentrations of 0.5 and 1 mM. H, HM1 and HM10 improved seed germination both in water and in the presence of Cu(2+). One or 10 microM melatonin eliminated the inhibitory effect of the 0.5 mM metal concentration on the fresh weight of seedlings. HM100 had a negative effect; thus seed germination was lower and seedlings had poor establishment. The toxic effect of Cu(2+) manifested by membrane peroxidation and DNA endoreplication blocking in the seedlings grown from nontreated (control) and H seeds was not observed in the seedlings grown from HM1 and HM10 seeds; in contrast, HM100 enhanced the toxic effect of Cu(2+).

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The localization of callose in the cell walls of megasporocytes and megaspores was studied by means of fluorescence microscopy. It was found that the part of the meiocyte cell wall in which callose begins to appear corresponds to the region of active megaspore formation. During the whole megasporogensis more of callose was found in walls of such cells which differentiate afterwards. For different species the pattern of callose distribution in walls during megasporogenesis is a constant specific trait.

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