Özet: D-Galaktoz (D-GAL), oksidatif stresi arttırarak doğal yaşlanma sürecini uyaran bir monosakkarittir. Bu çalışmada, yaşlanma ile ilişkili hasarlı modelde oksidatif stres üzerine günlük kefir tüketiminin etkisi araştırıldı. Sıçanlara 8 hafta boyunca D-GAL (300 mg/kg/5 gün), Düşük doz Kefir (DKFR) (0.7 ml/kg/5 gün) ve Yüksek doz Kefir (YKFR) (3.5 ml/kg/5 gün) uygulandı. Bu süre sonunda elde edilen beyin dokusu örneklerinde Glutatyon (GSH) ve Lipid peroksidasyonu (LPO) düzeyleri ile Süperoksit dismutaz (SOD) ve Asetilkolin esteraz (AChE) aktiviteleri ölçüldü. Kontrol grubuna kıyasla D-GAL grubunda GSH düzeyleri ve SOD aktivitelerinde anlamlı bir azalma gözlendi, ancak LPO düzeyleri ve AChE aktiviteleri arttı (p <0.05). Kefirin her iki dozunda da D-GAL verilen gruba kıyasla LPO düzeyleri azaldı (p <0.05). D-GAL grubuna kıyasla, D-GAL verilen düşük doz kefir grubunda SOD aktivitesi arttı, AChE aktivitesi azaldı (p <0.05). Aynı zamanda histopatolojik bulgular da kefirin taklitçi yaşlanma modelinde koruyucu bir etki göstererek oksidatif stresi azalttığını destekledi. Bu sonuçlar kefirin özellikle beyin dokusunda yaşlanma ile uyarılmış oksidatif streste faydalı etkilere sahip olabileceğini gösterdi. Abstract: D-Galactose (D-GAL), is a monosaccharide that mimics the natural aging process in rodents by increasing the oxidative stress. We investigated the effect of daily kefir consumption on oxidative stress caused in aging related damage model. Rats received D-GAL (300 mg/kg/day, 5 days), low dose of kefir (0.7 ml/kg/day, 5 days) and high dose of kefir (3.5 ml/kg/day, 5 days) during 8 weeks. At the end of the 8 weeks period, obtained in brain tissue samples Glutathione (GSH) and Lipid peroxidation (LPO) levels, Superoxide dismutase (SOD) and Acetylcholinesterase (AChE) activities were measured. The control group was compared with the D-GAL group, a significant decrease in GSH levels and SOD activities were observed, but LPO levels and AChE activities were increased (p <0.05). Both doses of kefir in D-GAL groups were decreased LPO levels as compared to D-GAL group (p < 0.05). The D-GAL group was compared with the D-GAL received low dose of kefir, a significant increase in SOD activity, a significant decrease in AChE activity were observed (p <0.05). At the same time, in histopathological findings of kefir have supported the decline of oxidative stress by showing a protective effect on the mimetic aging model. These results indicated that kefir may have some favorable effects on aging-induced oxidative damages in especially brain tissue. The Effect of Kefir on
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