A polycystic ovarian follicle (PCOF) syndrome associated with high baseline concentrations of progesterone (P4) without preovulatory luteinizing hormone (LH) surges has been reported in turkey hens. The PCOF syndrome could be induced in turkey hens by injecting P4 (0.33 mg/kg per d) daily early in the reproductive period for 10 to 12 d and then waiting 3 wk for the syndrome to develop. It was hypothesized that an arrest in laying associated with the PCOF syndrome could be induced by daily injection of P4 in restricted-fed broiler breeder hens. Hens were divided into 5 treatment groups and were injected subcutaneously daily with P4 in canola oil at dosages of 0, 0.17, 0.33, 0.5, and 1.5 mg/kg per d for 13 d, at 14 wk of egg production when they were 41 wk of age. Blood samples were collected on d 7 and 13 immediately before P4 injection. Oviductal and ovarian morphologies were measured at necropsy 1 d after the last P4 injection. Egg production rate was reduced by injection of P4 at dosages < 0.17 mg/kg per d. At dosages of 0.5 and 1.5 mg/kg per d, ovarian hierarchical follicles had regressed. None of the broiler breeder hens had the PCOF syndrome at necropsy, but a high incidence of hens holding hard-shelled uterine eggs for several days was observed. Concentrations of LH decreased with P4 injection at > 0.17 mg/kg per d, and P4 concentrations were increased with P4 injection at > 0.5 mg/kg per d. Estradiol-17beta (E2) concentrations were decreased at all P4 dosages. It was concluded that egg production rate was reduced by daily injection of P4 at dosages > 0.17 mg/kg per d, and egg production ceased and ovarian follicles and the oviduct regressed at dosages > 0.50 mg/kg per d. The PCOF syndrome, however, was not induced in restricted-fed broiler breeder hens by P4 injection.

In turkey hens, the egg production rate is relatively high early during a reproductive period, but declines as the period progresses. Among lines with different egg production potential, the interval between preovulatory surges of LH is the primary determinant of the egg production rate. The main objective of this study was to determine whether the decline in egg production rate late during an egg production period is also associated with a difference in the interval between LH preovulatory surges. A group of photosensitive turkey hens (Early) were photostimulated with continuous light (24L:0D) at 40 wk of age to induce egg laying, and serial blood samples were collected after about 3 wk of egg production. A second group of hens (Late) were housed in floor pens and photostimulated with 14L:10D at 40 wk of age for a normal 36-wk reproduction period and were then switched to 24L:0D lighting for 2 wk before collection of serial blood samples. Continuous light photostimulation was used for at least 2 wk before and during serial blood sampling to avoid potential masking effects of diurnal lighting on the interval between LH surges. The Early (n = 12) and Late (n = 16) hens were cannulated 3 days before being serially bled hourly for 10 days. The mean interval between preovulatory surges of LH was shorter in the Early hens than in the Late hens (26.1 +/- 2.5 h and 34.7 +/- 3.9 h, respectively). The intra-hen LH surge interval coefficient of variation was lower in the Early hens than in the Late hens (7.2% and 18.6%, respectively). The inter-hen LH surge interval coefficient of variation was similar in the Early and Late hens (9.5% and 11.2%, respectively). The incidence of blind surges of LH (those not retrospectively associated with ovipositions) was not different between Early and Late laying hens (8.4% +/- 15.2% and 7.3% +/- 14.6%, respectively). In conclusion, in turkey hens, longer intervals and greater intra-hen variation between LH surges were associated with a poorer rate of egg production late in the reproductive period relative to early in the reproductive period.