Streptomyces, Nocardia, and Pseudomonas capable of lysing hyphae of Fusarium solani or Neurospora crassa were obtained by selective culture, but attempts to isolate an organism lysing Rhizoctonia solani failed. When provided with F. solani or N. crassa as carbon sources, the actinomycetes tested produced f3-(1-* 3) glucanase and chitinase. A mixture containing purified chitinase and (3-(1-, 3) glucanase induced spheroplast formation in F. solani, caused some morphological changes in N. crassa, but had almost no effect on R. solani hyphae. The polysaccharides in R. solani walls, which contain a large amount of glucose as well as galactose, mannose, and glucosamine, were not hydrolyzed appreciably by the two enzymes. Laminaribiose and laminaritriose were released by enzymatic hydrolysis of F. solani and N. crassa walls, and gentiobiose was liberated from R. solani and N. crassa walls. Melaninlike materials were found in R. solani walls, accounting for
Activity of bacterial Llrease in the presence of three divalent cations
Mn2+MgZ+ Ca2+Urease activity as % of that of controls without S o~~r c e of urease added cation* Bacillus pastel~rii Brevibaderiz~nz antvjoniagenes MZC~OCOCCZLS ureae Proleus vztlgaris Runlen isolate 10D *Concentration of each cation: 1.2 X 10-2 molar. tested, however, suggest that differences in constitution exist between the ureolytic enzynies produced by different organisms. Support for this conclusion is provided by the finding of Yall and Green (5) that the susceptibility of StapJzylococczis attreus urease to inhibition by sulfydryl reagents varied with the conditions under which the organism was grown. This suggests t h a t S. aureus can produce urease with varying affinities for sulfydryl inhibitors.
A total of 134 alpha-amylase producing Bacillus isolates and 21 reference strains were divided into 12 groups according to their similarities (% SSM). Phenotypic characteristics determined by the API 20E and API 50CHB galleries, other biochemical tests and morphological characteristics were used for the numerical analysis. The API Computer Service identified 45% of the isolates. The amylase yields of 16 alpha-amylase hyperproducing (AHP) isolates were compared with those of seven amylolytic reference and type strains. The AHP isolates were related to Bacillus subtilis, B. licheniformis and 'B. amyloliquefaciens'.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.