Surges of luteinizing hormone (LH) in serum that result in luteinization, but occur prematurely with respect to the diameter of the leading follicle, frustrate attempts to induce multiple follicular maturation for in-vitro fertilization (IVF) in a number of women. We examined the possibility of blocking premature LH surges by the administration of D-TRP6-LH-RH, a potent agonistic analogue of gonadotrophin-releasing hormone (GnRH). Six patients who had repeatedly shown premature LH surges were treated for 10 days, beginning between days 1 and 3 of the cycle with daily s.c. injections of 500 micrograms D-Trp6-LH-RH followed by a daily injection of 100 micrograms of the analogue until the day of administration of human chorionic gonadotrophin (HCG). When pituitary and ovarian suppression had occurred, ovarian stimulation with human menopausal gonadotrophin was started and adjusted in dose according to the ovarian response. HCG was injected when the dominant follicle had reached a diameter of at least 18 mm and oestradiol levels were above 300 pg for each follicle greater than 15 mm. Oocyte collection was performed 36 h later via laparoscopy, followed by IVF and embryo transfer. The six patients studied to date responded to therapy and treatment could be completed up to embryo transfer. Two patients became pregnant; one of the pregnancies, however, resulted in abortion. Combined treatment with GnRH analogue for suppression of pituitary gonadotrophin secretion followed by the administration of gonadotrophins thus seems to be a promising method for ovarian stimulation in patients who frequently exhibit premature LH discharges and therefore fail to complete treatment.
Absolute cross sections for K-shell ionisation in thin foils of Ag and Cu by electron impact have been determined from NaJ(T1) scintillation spectrometer measurements of K-x-rays and with the aid of a fl-ray sp~trometer. The energies of the bombarding electrons have been 114 keV for Ag and 81 keV, 114 keV and 152 keV for Cu. The results for Cu are in excellent agreement with the relavistic calculations of Arthurs and Moiseiwitsch. A brief discussion of the values obtained in comparison with earlier experimental and theoretical results will be given.
Gonadotrophin and testosterone levels were measured in male foetal rats at the 21st day of gestation 30 min following administration of LRH. The releasing hormone was administered by 3 different routes: subcutaneously as a single injection to the pregnant rat; subcutaneously as 12 times repeated injection to the pregnant rat from 16th-21st day of gestation; direct injection into the amniotic cavity. Three different doses of 1, 5, and 10 microgram/kg were used. The single administration to the dam was without effect. Repeated doses resulted in a significant dose-dependent elevation of mean foetal LH: 163 ng/ml in the control animals and 133,256, and 363 ng/ml in the 1, 5, and 10 microgram/kg LRH group resp. Intra animal injections significantly increased FSH and LH levels, but only those of FSH were clearly dose-dependent. A mean of 390 ng/ml in the control group was observed, with 1 microgram/kg LRH FSH was elevated to 723 ng/ml, at 5 microgram/kg to 928 and at 10 microgram/kg to 1017 ng/ml. Testosterone levels were not significantly altered. Our results demonstrate that the pituitary of foetal rats is able to respond to LRH in the same manner as adult animals.
The angular dependence of cross sections with state analysis has been measured for the K–Hg interaction with collision energies of 100 and 200 eV. Two experiments have been performed: one with particle-photon coincidence to yield fine structure resolution of the K(42P) inelastic channel and one to resolve the P3/2 component with respect to the absolute value of mj.
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