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This study contributes to further characterize PPPK-BFB in consanguineous families and to extend the mutational spectrum of AAGAB gene in the Tunisian population.

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Bluetongue (BT) is an arboviral disease that affects ruminants. BT virus is transmitted by biting midges of the genus Culicoides (Diptera: Ceratopogonidae). Since 1999, BTV outbreaks have occurred in Tunisia and two serotypes (BTV-2 and BTV-1) have been involved in 2000 and 2006, respectively. Entomological surveillance has been implemented in Tunisia with the aim to study population dynamics of C. imicola and other potential vectors. Two-night catches of midges per site were performed monthly from June 2006 through July 2008 on 14 sites through­out the country. A total of 11,582 Culicoides specimens, col­lected from 336 light traps, comprised 25 species of which seven were identified for the first time in Tunisia. The seasonal occur­rence of these species is described.

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