Capillary radioimmunoassay -- a modification of the coated tube technique -- is described. The assay is equivalent (accuracy, sensitivity, precision) to conventional methods for insulin determination; it overcomes, however, the necessity of centrifugation or filtration and offers the added bonus of being automation compatible.
A new modification of the "coated-tube"-technique for the radioimmunoassay of human placental lactogen is described. Polyvinylchloride capillaries are used as reaction-vessels. Most working steps of the test procedure are mechanized. The possibilities and limitations of the new technique are discussed. The reliability of the method was shown by a recovery test, dilution tests, reproducibility and a comparison of the results for 71 sera, ranging from the 6th to the 42nd week of pregnancy, with those obtained by a conventional assay procedure.
Kombinierte batchwise und continuous flow-Mechanisierung von Festphasen-RadioimmunoassaysZusammenfassung: Es wird eine neue Modifikation der "coated-tube"-Technik am Beispiel der radioimmunologischen Human Placenta Lactogen-Bestimmung beschrieben. Reaktionsgefäße sind Kapillaren aus Polyvinylchlorid. Die Durchführung des Tests ist weitgehend mechanisiert. Die Arbeitstechnik wird beschrieben, Möglichkeiten und Grenzen der Technik werden diskutiert. Wiederfindung, Verdünnungsreihen, Reproduzierbarkeit, sowie ein Methodenvergleich an 71 Schwangerenseren zeigen die Zuverlässigkeit der Methode.
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