During deep fat frying process, several compounds arise in the oil. The determination of their quantity by the classical methods is a very long and laborious work. For this purpose, quick instrumental examinations are needed. Therefore, the authors have investigated the possibilities of applicability of the NIR technique for determining the polar, polymer and linoleic acid contents of the sunflower oil heated at 170-18O0 C and 200-210O C, resp., for different times. They examined the equation forms describing the relationship between the parameters and values measured at given wavelengths of the NIRspectra, the characteristic wavelengths, the Kvalues and the accuracy of the determination.They established that the NIR technique can give successful results by the quick determination of the examined parameters. It was found that the standard error of calibration (SEC) of polar content at a thermal load of 170-180O C was 0.991"/n and at that of 200-21O0C 0.9150/0, that for polymer content 1.21"/0 and 1.65"/0, resp., that for linoleic acid content 0.436'b and 0.446 "Yo,resp., and the multiple correlation coefficient was ranging between 0.994 and 0.999.
The present study was conducted to identify and quantify the phenolic profile of parsley seeds and green parts methanolic extracts, to investigate the essential oil composition extracted from parsley seeds, to purify bioactive components from green parts of ethyl acetate fraction and to investigate the antimicrobial activity of this mentioned preparations. Results revealed that parsley seeds methanolic extract contained higher amount of polyphenols than leaves methanolic extract. Rosmarinic acid was the most detected compound in parsley seed methanolic extract carried out by HPLC technique (38777 µg/g) meanwhile in green parts methanolic extract (as 21468 µg/g). Apiole was the main constituent of the essential oil of parsley seeds (45.21 %). Ethyl acetate fraction of the green parts was employed to TLC chromatographic analysis. The component of the main band was purified with preparative thick layer chromatogram PTLC using ethyl acetate: methanol: water (100:16.5:13.5 by volume) as running system. The obtained data from NMR spectrum for the purified component showed the presence of Kaempferol 3arabinofuranoside. Seeds and green parts methanolic extracts were evaluated as antimicrobial agent along Kaempferol 3-arabinofuranoside in a comparative study with seeds essential oil. Aromatic oil of Petroselinum crispum was the most effective against Escherichia coli, Staphylococcus aureus , Salmonella typhi. Fusarium oxysporum, and Alternaria alternate being with inhibition zones 25.6 , 23.8 , 30.0 30.22 and 25.64 mm, respectively. Kaempferol 3-arabinofuranoside demonstrated moderated antibacterial activity against the examined microbes.
Hypoglycemic action of methanolic extracts of Bauhinia variegata and Enterolobium cyclocarpum leaves were studied on streptozotocin diabetic rats. Two different doses(200 and 400 mg/kg body weight)of the extracts were given orally to the rats for 22 days. Lipid profile improvement and hepatoprotective effects against liver disorders associated with diabetic complications were also studied .Results revealed that Bauhinia variegata extract showed a strong antidiabetic activity at the two doses. The doses of 400mg/kg b.wt. showed the highest activity. Enterolobium cyclocarpum extract at the two doses failed to decrease blood glucose level to the healthy range. The mode of Hypoglycemic action of Bauhinia variegata extract may due to the presence of considerable amounts of Flavonoids and polyphenols. The two extracts (Bauhinia variegata and Enterolobium cyclocarpum) could improve lipid profile of diabetic rats and Enterolobium cyclocarpum had weaker ability. Bauhinia variegata methanolic extract resulted in significant decrease in liver malondialdehyde (MDA)and increase in reduced glutathione (GSH) .
Trypsin inhibitor activity was determined in both fresh and dry seeds of four legumes namely; broad bean, cowpea, peas and kidney bean. The obtained results showed that the values of trypsin inhibitor activity (TIA) for all samples were higher in dry seeds than those in the corresponding fresh samples.Dry kidney bean had the highest level of TIA (13.90 TUI/mg) followed by cowpea (12.6 TUI/mg) and pea (9.10 TUI/mg).Germination of the legume seeds resulted in progressive decrease in TIA. After 72 hrs of germination, more than 60% of TIA were declined in all samples. Extending germination period to 96 hrs, slightly affected the retained activity of trypsin inhibitor in all samples.Boiling dry faba bean seeds in water for 3 hrs, remarkably decreased TIA. The remained activity was 28% of the original TIA in the untreated sample. Boiling the other legume seeds for different periods resulted in a sharp decrease in TIA in all samples after boiling for 20 min. More than 70% of TI was inactivated, in all samples, after boiling for 60 min.Neither germination nor boiling caused complete removal of TIA. Therefore, a combination of the two treatments might be more effective.
Three vegetable oils (virgin olive, crude canola, or linseed oils) were used as diet additives to compare their effects on lipid profile of blood serum of experimental rats in the presence of egg yolk as a source of cholesterol. Corn meal (1.6% fat) was used as control diet, the positive control diet composed of corn meal plus 9% egg yolk (about 0.15%cholesterol). The other three diets composed of positive control plus 14% olive, canola or linseed oils. Lipid parameters were determined in rat's blood serum after 21, 36 and 46 days from feeding . Obtained data revealed that both canola and linseed oils containing diets significantly decreased total cholesterol (TC), triacylglycerol(TG) and low-density lipoprotein cholesterol (LDL-c) and increased highdensity lipoprotein cholesterol (HDL-c) levels in relation to positive control diet. Data of olive oil containing diet showed a significant increase in TC, TG, and HDL-c at the end of nutrition experiment. Whereas no significant difference in LDL-c between positive control and olive oil containing diet.
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