629th MEETING. LONDON 743 6o 50 I , X "0 1 2 3 1 5 Time (h) Fig. 1. Substrate incorporation by tegument membrane fraction Tegument membrane fraction was incubated with [3H]NAD (O), [3H]adenosine ( * ) and [3H]hypoxanthine ( A ). Samples(triplicate) were incubated for 5 h at 26°C and radioactivity was measured in acid-precipitable material after the addition of 20% (v/v) trichloroacetic acid at 4°C. moreover, inclusion of non-radioactive AMP and adenosine over the concentration range of 10-100 p~ inhibited the uptake of ["HINAD. Inosine and hypoxanthine were both found to inhibit the incorporation of [3H]adenosine into acidinsoluble materials due to isotope dilution and incorporation of the cold substrate. Incorporation of the radioactive substrates was not affected when uric acid (100 p~) was
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