Background: Prevalence of Helicobacter pylori (H. pylori) differs among populations worldwide. H. pylori has been correlated with gastritis, peptic ulcer and cancer. Considering the treatment failure due to antibiotic resistance, proper treatment of H. pylori infection is of great importance. Objectives: The aim of this study was to determine the prevalence of H. pylori infection based on age, sex and also to examine antibiotic resistance among patients referred to the endoscopy department of Shahid Beheshti University of Medical Sciences hospitals in Tehran from 2010 to 2011. Patients and Methods: In this descriptive study, biopsies were taken from 192 patients with dyspepsia who underwent endoscopic evaluation. For diagnosis of H. pylori infection and histologic assessment, samples were stained by Giemsa and hematoxylin-eosin (H & E) staining. Based on the Sydney system for classification of gastritis, extent of H. pylori infection and severity of gastritis were measured. To determine antibiotic resistance after culturing on Brucella agar medium, antibiogram test was done using modified disk diffusion method on Mueller-Hinton agar considering standard conditions. Data analysis was done by the SPSS 16.0 software, using the chi-square and T-test methods. Results: Overall prevalence of H. pylori was 83% among all patients and 90.3% among those with gastritis (CI 95% = 85.9%-94.6%). There was no significant correlation between the extent of H. pylori infection and age (P = 0.182) and gender (P = 0.466). Yet, there was a significant correlation between H. pylori infection and severity of gastritis (P = 0.001). Rates of resistance to metronidazole, clarithromycin, amoxicillin, ciprofloxacin and tetracycline were 50%, 16.1%, 5.2%, 4.6% and 3.6%, respectively. Conclusions: Prevalence of H. pylori infection in our patients was similar to that of developing countries. Severity of gastritis is correlated with the extent of H. pylori infection. Moreover, the antibiotic resistance rate observed in this study signifies the ever-growing importance of further antibiotic sensitivity studies to help with proper treatment regimens against H. pylori.
Article Subject: Medical Bacteriology 10.30699/ijmm.13.3.164 Background and Aims: The type IV Pilin is an important colonization factor for opportunistic pathogens of Pseudomonas aeruginosa, which plays a role in the formation of biofilms and binding to the host cells. Each type of Pilin is coded with a particular auxiliary gene. This specific relationship can be used as a therapeutic target for detecting P. aeruginosa strains as well as its molecular classification. The purpose of this study was to evaluate the frequency of different types of auxiliary genes in cystic fibrosis, burns, and environmental samples. Materials and Methods: Pseudomonas aeruginosa samples were collected from patients with cystic fibrosis, burns as well as environmental wastewaters during 2016-2017. Samples were cultured and identified using standard microbial and biochemical methods. DNA extraction was performed by boiling and PCR was performed through specific primers. Results: Totally, 90 isolates of P. aeruginosa samples (35 environmental, 30 burns, and 25 cystic fibrosis) were examined. tfpO and tfpZ were positive in 71 and 2 isolates, respectively. Conclusion: The results indicated that Pseudomonas aeruginosa Pilin types are very diverse. Regardless of the source of the samples, the most common tfp was tfpO. Taking into account the fact that tfpZ was found only in burns, it can be assumed that this particular type may appear in severe clinical conditions. Ultimately, larger statistical population and use of more comprehensive typing methods is suggested for better results.
FN (70, 80, 90 and 100 ug/ml) and the adherence was visualized by immunofluorescence using hyperimmune rabbit sera against T. solium activated oncospheres. In the other hand, only the CHO -K1 cells were preincubated with 80 ug/ml of FN for 20 h and then the T. solium oncospheres with and without FN were added on monolayer cell. FN fibrils and oncospheres were visualized by immunofluorescence using polyclonal anti-FN antibody produced in rabbit by UV light microscopy.Results: The adherence of T. solium oncospheres was increased from 70 ug/ml FN versus control. The fibrillar FN matrix on CHO-K1 cells were visualized over monolayer cell. Also, the adherence T. solium oncosphere with FN preincubate reacted with anti-FN antibody but with minor intensity versus control.Conclusion: These results suggest that Fn increases the adherence of oncosphere of T. solium serving as a bridge between the T. solium oncosphere and cell. Additonal characterization of T. solium oncosphere fibronectin binding protein that interact with host components, will further our understanding of the adherence and could be markers as candidate a vaccines.
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