SUMMARY
Three clones of the diatom Amphora were euryhaline, able to grow autotrophically at 160 lx (0.001 ly/min) and heterotrophically on glucose and fructose. Furthermore 2 clones grew on glutamate and feast extract. Light‐limited growth of individual clones was stimulated by glycerol, galactose, lactate, acetate, aspartate and asparagine, although mannose torn inhibitory at low and high light levels. The half‐saturation constant for growth of A. coffeaefomis var. perpusilla Grunow (Cleve) with glucose was 25 μM. Heterotrophic growth rate of this organism became saturated with respect to glucose at 0.5 mM.
Amphora coffeaeformis (Ag.) Kütz. var. perpusilla (Grun.) Cleve took up glucose by an inducible transport system. The system was induced by d‐fructose, d‐mannose, as well as glucose. Some d‐pentoses also induced a glucose uptake system but it may not be the same one as that induced by hexose. d‐fructose, d‐mannose and 2‐deoxy‐d‐glucose inhibited 2 mM glucose uptake at equimolar concentration, but d‐pentoses did not. The uptake system decayed in ca. 5 h in the absence of glucose. The half‐saturation constant for uptake, K8 was ca. 0.1 mM glucose with a maximum uptake rate, Vmax= 0.4 nmol/106 cells‐min−1.
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