favorable case. We could not go ourselves to collect the blood, but Dr. Hyatt kindly collected about 5 c.c in sterile sodium citrate solution (1.5 per cent, furnished by us). Some of the blood had been discharged into tubes of plain broth (made by Dr. Hyatt). Two of these inoculated broth tubes were turned over to us for observation. The citrated blood was centrifuged. The citrate plasma was removed and the sediment hemolyzed with distilled water. The hemolyzed sediment and plasma were then centrifuged at high speed for one hour and the sediments planted in deep tubes of 4-0.3 and 4-1.0 Martin's medium containing sterile pleu¬ ritic fluid. Two plates of combined sediments were made ; one was kept aerobic and the other at partial tension at 37 C. All the cultures showed no growth during two weeks' observation.
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