Study design: An animal model of transected spinal cord injury (SCI) was used to test the hypothesis that cografted neural stem cells (NSCs) and NT-3-SCs promote morphologic and functional recoveries of injured spinal cord. Objective: To explore whether cotransplant of NSCs and NT-3-SCs could promote the injured spinal cord repair. Setting: Zhongshan Medical College, Sun Yat-sen University, PR China. Methods: Female Sprague-Dawley (SD) rats weighing on 200-220 g were used to prepare SCI models. The spinal cord was transected between T 9 and T 10 , then NSCs, SCs þ NSCs, LacZSCs þ NSCs, or NT-3-SCs þ NSCs were grafted into the transected site. Results: (1) Part of NSCs could differentiate to neuron-like cells in the transected site and the percentage of differentiation was NT-3-SCs þ NSCs group4SCs þ NSCs group4NSCs group. (2) In the grafted groups, there were 5-HT, CGRP, and SP positive nerve fibres within the transected site. Some fluorogold (FG)-labeled cells were found in the spinal cord rostral to the transected site, the red nuclei and the inner pyramidal layer of sensorimotor cortex. (3) The cells grafted could enhance the injured neurons survival in inner pyramidal layer of sensorimotor cortex, red nuclei of midbrain, and Clark's nuclei of spinal cord's L1 segment, could decrease the latency and increase the amplitude of cortical somatosensory evoked potential (CSEP) and cortical motor evoked potential (CMEP), and could promote partly structural and functional recovery of the SCI rats.Conclusion: These results demonstrate that cografted NT-3-SCs and NSCs is a potential therapy for SCI.

To reveal the complex impact of the road grade on heavy-duty diesel truck (HDDT) emissions, the dynamic coupling relationships among the road grade, vehicle operation, and emissions were quantified based on portable emission measurement system data from 24 HDDTs in Chongqing, China. The results showed that the average emission factors of nitrogen oxides (NO x) and carbon dioxide (CO2) of China IV HDDTs were the highest among all tested HDDTs, and NO x emissions often exceeded their corresponding standard limit values. Next, the coupling relationships of road grade, speed, and emissions were studied. Graded roads caused a 4%–44% decrease in the average vehicle speed, resulting in a coupling effect on emissions. For all pollutants and vehicle emission standards, the HDDT emissions on uphill roads were much higher, while those on downhill roads did not deteriorate significantly owing to the increased acceleration operation. Overall, ignoring the road grade led to estimation errors of between −99.14% and 291.30% for CO2 and between −99.21% and 247.73% for NO x. Speed was less correlated with emissions on downhill roads (≤−3%). For uphill roads (>0%), the joint effect of road grade and speed caused a significant increase in CO2 and NO x emissions up to 21.26 and 17.29 times compared to idling emissions. A Sigmoid function successfully modeled the coupling relationship (average R2 = 0.95), and grouped S-shaped mapping curves were revealed for between −8% and 7% road grade and emissions. The model can estimate the influence of road grades on emissions at different speeds, thus providing support for emission reduction control.