The stimulation of neutrophils by formylmethionyl-leucyl-phenylalanine results in a bimodal luminol-dependent chemiluminescence pattern. We observed, however, only a single peak chemiluminescence pattern in the response to the peptide when we used lucigenin as an amplifying substance. We suggest that lucigenin, the larger molecule, (510 daltons; luminol is 177 daltons) only exerts an extracellular effect.
Phorbolmyristate acetate (PMA) induced both a luminol‐ and a lucigenindependent chemiluminescence response in normal polymorphonuclear leukocytes (PMNL). PMNL isolated from a patient with a myeloperoxidase (MPO)‐deficiency were found to produce almost no luminol‐dependent chemiluminescence. The lucigenin‐dependent chemiluminescence response was, however, found not to differ between normal and MPO‐deficient cells. From these results it was concluded that the luminol‐ and the lucigenin‐dependent chemiluminescence differed in the dependence of MPO. Results obtained with cell‐free MPO‐H202‐ and superoxide anion producing systems further supported this conclusion.
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