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Effect of preparation conditions on structural and magnetic properties of Mn and Mn-Ga spinels was investigated by Mössbauer spectroscopy and magnetization measurements. These properties were found to be significantly dependent on the quenching temperature of the samples. Evidently, quenching from elevated temperatures enhanced the magnetization of the spinels due to changes in the chemical short range order.

Myeloid‐derived suppressor cells (MDSC) play a crucial role in controlling T‐cell responses, but their development and suppressor mechanisms are not fully understood. To study the molecular functions of MDSC, a large number of standardized cells are required. Traditionally, bone marrow (BM) has been used to generate myeloid cell types, including MDSC. In this study, we demonstrate that a previously described protocol for generating monocytic MDSC (M‐MDSC) from murine BM with GM‐CSF can be fully transferred to BM cells that are conditionally transformed with HoxB8 gene (HoxB8 cells). HoxB8 cells have an extended lifespan and efficiently differentiate into MDSC that are quantitatively and qualitatively comparable to M‐MDSC from BM cells. Flow cytometric analyses of LPS/IFN‐γ activated cultures revealed the same iNOS+ and/or Arg1+ PD‐L1high M‐MDSC subsets in similar frequencies from BM or HoxB8 cells. In vitro suppression of CD4+ and CD8+ T‐cell proliferations was also largely comparable in their efficacy and its iNOS‐ or Arg1‐dependent suppressor mechanisms, which was confirmed by the similar amounts of nitric oxide (NO) secretion measured from the suppressor assay. Therefore, our data suggest that murine M‐MDSC generation from HoxB8 cells with GM‐CSF can be used to substitute BM cultures.