Three patients had subcutaneous nodules at the sites of previous injections of vaccine containing tetanus toxoid. Biopsy and microscopic findings in all three cases showed foci of granulomatous inflammation, consisting of lymphoid follicles, in the deep dermis and subcutaneous tissues and a surrounding infiltrate of lymphocytes, histiocytes, plasma cells, and eosinophils. Occasional zones of eosinophilic necrosis were seen within the inflammatory foci, and surrounding dense fibrous bands were present in some areas. A solochrome-azurin stain showed aluminum crystals in the nodules from two patients. From the evidence available, we believe that these nodules are a complication of inoculations with aluminum-containing vaccines.

Plasma membrane-and Golgi vesicle-enriched membrane fractions were prepared from day-10 lactating rat mammary glands. Each fraction was found to contain a single set of D-glucose-inhibitable cytochalasin B-binding sites: plasma membranes and Golgi vesicles bound 20+ 2 and 53 +4 pmol of cytochalasin/mg of membrane protein (means + S.E.M.), with dissociation constants of 259 + 47 and 520 + 47 nm respectively. Anti-peptide antibodies against the C-terminal region (residues 477-492) of the rat brain/human erythrocyte glucose transporter labelled a sharp band of apparent Mr 50000 on Western blots of both fractions. Treatment with endoglycosidase F before blotting decreased the apparent Mr of this band to 38000, indicating that it corresponded to a glycoprotein. Confirmation that this immunologically cross-reactive band was a glucose transporter was provided by the demonstration that it could be photoaffinity-labelled, in a D-glucosesensitive fashion, with cytochalasin B. Quantitative Western blotting studies yielded values of 28 + 5 and 23 + 3 pmol of immunologically cross-reactive glucose transporters/mg of membrane protein in the plasma membrane and Golgi vesicle fractions respectively. From comparison with the concentration of cytochalasin B-binding sites, it is concluded that a protein homologous to the rat brain glucose transporter cons'titutes the major glucose transport species in the plasma membranes of mammary gland epithelial cells. Glucose transporters are also found in the Golgi membranes of these cells, at least half of them being similar, if not identical, to the transporters of the plasma membrane. However, their function in this location remains unclear.

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Ten male patients aged 38-63 years are presented, in whom a chronic, itchy, papular eruption developed on the upper trunk. Histology showed the characteristic features of Darier's disease. All the patients had clinical evidence of considerable solar damage and skin malignancies were frequently present. The condition is thought to represent a distinct entity which has been called persistent acantholytic dermatosis. The relationship between this condition, transient acantholytic dermatosis and actinic keratoses with Darier-like histology is discussed.