SummarySuccessful packing of high quality HPLC columns is dependent upon a number of parameters. Especially when using slurry packing techniques, the absence of conglomerates of particles in the slurry and the wettability of the particles are important. The method described in this paper fulfills these conditions, imparting a t; potential to the 5 and 10 pm silica particles by applying a pH gradient. This results in repulsive forces between the particles and hence leads toa well-controlled monodisperse situation. Water is used as a slurry and displacement liquid; it combines the advantages of excellent wetting properties and non-toxicity.
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