No abstract

Despite having ligands with almost identical conformations, the structures of the AgI complexes of 1 and 2 differ significantly: ligand 1 forms a double‐helical complex of P chirality, and 2 gives a triple‐helical complex (depicted on the right). The reason for the difference appears to be the existence of strong interligand stacking in the latter case.

A large number of studies demonstrate that cell mechanics and pathology are intimately linked. In particular, deformability of red blood cells (RBCs) is key to their function and is dramatically altered in the time course of diseases such as anemia and malaria. Due to the physiological importance of cell mechanics, many methods for cell mechanical probing have been developed. While single-cell methods provide very valuable information, they are often technically challenging and lack the high data throughput needed to distinguish differences in heterogeneous populations, while fluid-flow high-throughput methods miss the accuracy to detect subtle differences. Here we present a new method for multiplexed single-cell mechanical probing using acoustic force spectroscopy (AFS). We demonstrate that mechanical differences induced by chemical treatments of known effect can be measured and quantified. Furthermore, we explore the effect of extracellular vesicles (EVs) uptake on RBC mechanics and demonstrate that EVs uptake increases RBC deformability. Our findings demonstrate the ability of AFS to manipulate cells with high stability and precision and pave the way to further new insights into cellular mechanics and mechanobiology in health and disease, as well as potential biomedical applications.

No abstract

Visualizing the spatiotemporal organization of plasma membrane proteins at the nanoscale by combining live-cell single-molecule applications with direct fluorescent dye labeling via genetic code expansion and bioorthogonal chemistry.