In the present investigation biomolecules in the blood of spider Crossopriza lyoni were determined in albino mice. For visualizing biomolecule effects, physiological or sub-lethal dose of purified venom toxins of spider Crossopriza lyoni was administered in laboratory reared albino mice. Level of serum total protein was decreased significantly (p<0.05) up to 78 % and 69 % at 6 h of 40% and 80% of 24-h LD50, respectively as compared to control. Later on, it was slightly recovered up to 85 % and 84 % at 10 h in comparison to control. Level of serum free amino acid was increased significantly (p<0.05) up to 117 % at 6 h of 40% of 24-h LD50 of purified Crossopriza lyoni venom toxins while 80 % of 24-h LD50 caused a significant increase of 151 % at 8 h. A significant (p<0.05) elevation in serum uric acid was found to be 138 % and 136 % at 8 h of treatment with 40 % and 80 % of 24-h LD50 of purified Crossopriza lyoni venom toxins, respectively in comparison to control. Similarly a marginal reduction in serum cholesterol level i.e. 87 % and 88 % was found at 10 h of treatment with 40 % and 80 % of 24-h LD50 of purified venom toxins from C. lyoni in comparison to control. Serum pyruvic acid increased significantly (p<0.05) up to maximum 164 % at 6 h of treatment with 40 % of 24-h LD50 of purified C. lyoni venom toxins in comparison to control. Similarly, a significant (p<0.05) elevation in serum glucose level i.e. 152 % was noted at 10 h of treatment with 40 % of 24-h LD50 of purified C. lyoni venom toxins in comparison to control.
In the present study, attempts were made to develop and evaluate the controlled porosity osmotic pump (CPOP) based drug delivery system of sparingly water soluble drug Baclofen. Formulation variables, such as, levels of solubility enhancer, ratio of drug to osmogents, coat thickness of semi permeable membrane (SPM) and level of pore former were found to affect the drug release from the developed formulations. Cellulose acetate was used as the semi permeable membrane. Drug release was directly proportional to the level of the solubility enhancer, osmotic pressure generated by osmotic agent and level of pore former; however, was inversely proportional to the coat thickness of SPM. Drug release from developed formulations was independent of pH and agitation intensities of release media. Burst strength of the exhausted shells decreased with increase in the level of pore former. This system was found to deliver Baclofen at a zero-order rate. The optimized formulations were subjected to stability studies as per ICH guidelines, and formulations were found to be stable after 45days study.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.