This 2-year field experiment investigated the effects of full straw incorporation on soil fertility and crop yield in a rice-wheat (Oryza sativa L.–Triticum aestivum L.) rotation on sandy, loamy soil. Two treatments were tested: (i) straw removal (CK) and (ii) straw incorporation (STR). The STR significantly increased the wheat yield by an average of 58% compared with CK; however, no significant difference was found in the rice yield. Soil available nitrogen, phosphorus, and potassium in the 0–20 cm soil layer increased by more than 15% with STR compared to CK. The soil cation exchange capacity and organic carbon in the 0–20 cm soil layer increased by 8% and 22%, for STR compared to CK, respectively. Straw incorporation significantly elevated the soil saturated water content but decreased the soil bulk density compared with CK. Soil aggregates >2 mm were significantly increased after straw return. STR also notably increased the soil urease, invertase, and catalase activities in the 0–15 cm soil layer by 11.4%, 41.0%, and 12.9%, respectively, and the soil microbial carbon and nitrogen contents in the 0–20 cm soil layer by 59% and 54%. Therefore, full straw incorporation could significantly improve soil fertility and maintain crop yields for the study area.

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